Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,635	G→A	A339T (GCA→ACA)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,635	0	G	A	100.0%	51.3 / NA	16	A339T (GCA→ACA)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/5);  total (11/5)

TTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTAT  >  NC_000913/78502‑78770                                                                                                                                      |                                                                                                                                        ttACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTa                                                                                                                                   >  2:383363/1‑140 (MQ=255)                  tCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAAc                                                                                                                  <  1:144531/140‑1 (MQ=255)                                aTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCgg                                                                                                    >  2:318285/1‑140 (MQ=255)                                                                tGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCaa                                                                    >  1:352287/1‑140 (MQ=255)                                                                            cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGtt                                                        >  2:330627/1‑140 (MQ=255)                                                                                     gggTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACt                                               <  2:306299/140‑1 (MQ=255)                                                                                        tATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTg                                            <  1:383363/140‑1 (MQ=255)                                                                                                 gCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACt                                   >  2:158323/1‑140 (MQ=255)                                                                                                    aTGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTggg                                >  2:177871/1‑140 (MQ=255)                                                                                                         ttCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAAtt                           <  1:306629/140‑1 (MQ=255)                                                                                                            aGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCg                        >  2:158851/1‑140 (MQ=255)                                                                                                                        cTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCtgtt            >  2:2917/1‑140 (MQ=255)                                                                                                                         tGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCtgttg           <  1:302008/140‑1 (MQ=255)                                                                                                                                 cGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCatta   >  2:185762/1‑140 (MQ=255)                                                                                                                                 cGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCatta   >  2:48372/1‑140 (MQ=255)                                                                                                                                 cGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACT‑GGTAATTGCGGTTATTTCTGTTGTCGCattat  >  2:144759/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTAT  >  NC_000913/78502‑78770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑