Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,211	G→A	A222V (GCT→GTT)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,211	0	G	A	100.0%	97.9 / NA	29	A222V (GCT→GTT)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/15);  total (14/15)

ACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGATTTCCTG  >  NC_000913/765088‑765344                                                                                                                            |                                                                                                                                      aCAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTc                                                                                                                       <  1:359954/140‑1 (MQ=255)        tGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACAcc                                                                                                                >  2:343864/1‑140 (MQ=255)          gCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCg                                                                                                              >  2:325257/1‑140 (MQ=255)             aaGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGAcc                                                                                                           <  1:343864/140‑1 (MQ=255)                ggCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATc                                                                                                        <  1:127479/140‑1 (MQ=255)                ggCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATc                                                                                                        <  1:51724/140‑1 (MQ=255)                ggCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATc                                                                                                        <  1:325257/140‑1 (MQ=255)                                     tGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATAc                                                                                   <  2:171331/140‑1 (MQ=255)                                             gTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCaa                                                                           >  2:248203/1‑140 (MQ=255)                                                   tgGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCg                                                                     >  2:101042/1‑140 (MQ=255)                                                      ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACTATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCt                                                                  >  2:70537/1‑140 (MQ=255)                                                      ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCt                                                                  >  1:363419/1‑140 (MQ=255)                                                      ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCt                                                                  >  2:331032/1‑140 (MQ=255)                                                      ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCt                                                                  >  2:296763/1‑140 (MQ=255)                                                               tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGc                                                         <  1:253501/140‑1 (MQ=255)                                                               tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGc                                                         >  2:297764/1‑140 (MQ=255)                                                                                  cGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACTATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCt                                      <  1:70537/140‑1 (MQ=255)                                                                                             cAGGGTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCgg                           <  1:248203/140‑1 (MQ=255)                                                                                               gggTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGtt                         <  1:296763/140‑1 (MQ=255)                                                                                               gggTAAATTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGtt                         <  1:331032/140‑1 (MQ=255)                                                                                                    aaTTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGt                          >  1:338642/1‑134 (MQ=255)                                                                                                    aaTTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGt                          <  2:338642/134‑1 (MQ=255)                                                                                                    aaTTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCAt                    <  1:55815/140‑1 (MQ=255)                                                                                                    aaTTTATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCAt                    >  1:227674/1‑140 (MQ=255)                                                                                                      tttATAGTCATCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCa                  >  2:72248/1‑140 (MQ=255)                                                                                                                tCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGAt        >  2:266562/1‑140 (MQ=255)                                                                                                                tCGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGAt        <  1:297764/140‑1 (MQ=255)                                                                                                                 cGGTATTAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGAtt       >  2:278948/1‑140 (MQ=255)                                                                                                                      ttAAAAACGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGATTTCCTg  <  1:101042/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                      ACAGGAATGGCGAAGGGCTGTCGGTTTCGACATGGTTGGCCATCGTATGATGGCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACCCGACCATCAACTAAATATCCACGCGATACTTTTTCAAGAATCGGCTTTGTCTGGCTGATATTAAGCAGACGGCTCATCTCTTCGGTTGGCATCAGAGGAATGATTTCCTG  >  NC_000913/765088‑765344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑