Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	830,184	C→T	A154T (GCC→ACC)	cecR ←	DNA‑binding transcriptional dual regulator CecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	830,184	0	C	T	100.0%	118.2 / NA	35	A154T (GCC→ACC)	cecR	DNA‑binding transcriptional dual regulator CecR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/18);  total (17/18)

TTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAG  >  NC_000913/830050‑830319                                                                                                                                       |                                                                                                                                        ttcttcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCaa                                                                                                                                    <  1:350240/140‑1 (MQ=255)  tcttcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAAt                                                                                                                                   >  2:340808/1‑140 (MQ=255)  tcttcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAAt                                                                                                                                   >  1:7528/1‑140 (MQ=255)     tcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAg                                                                                                                                <  1:165066/140‑1 (MQ=255)     tcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAg                                                                                                                                <  2:310950/140‑1 (MQ=255)      cATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAgg                                                                                                                               >  1:281707/1‑140 (MQ=255)             cgcgGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCa                                                                                                                        <  1:340808/140‑1 (MQ=255)                  tCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTgg                                                                                                                   <  1:171178/140‑1 (MQ=255)                    cAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCt                                                                                                                 <  2:352062/140‑1 (MQ=255)                            aCGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGTCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCg                                                                                                         >  2:354213/1‑140 (MQ=255)                            aCGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCg                                                                                                         >  1:291292/1‑140 (MQ=255)                                   aGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAAt                                                                                                  >  1:230972/1‑140 (MQ=255)                                     aaTTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCa                                                                                                >  1:298527/1‑140 (MQ=255)                                          tttCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCAcc                                                                                              <  2:98173/137‑1 (MQ=255)                                          tttCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCAcc                                                                                              >  1:98173/1‑137 (MQ=255)                                               ttGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTg                                                                                      >  1:87234/1‑140 (MQ=255)                                                  ccAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCAc                                                                                   <  2:281707/140‑1 (MQ=255)                                                   cAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCAcc                                                                                  <  2:291292/140‑1 (MQ=255)                                                     aGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGTCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAg                                                                                <  1:354213/140‑1 (MQ=255)                                                            aCGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGTCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAg                                                                         >  2:366603/1‑140 (MQ=255)                                                            aCGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAg                                                                         >  1:261167/1‑140 (MQ=255)                                                                cAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTg                                                                     <  2:7528/140‑1 (MQ=255)                                                                   aaTCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCg                                                                  <  1:362486/140‑1 (MQ=255)                                                                        cACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGc                                                                    >  1:93870/1‑133 (MQ=255)                                                                        cACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGc                                                                    <  2:93870/133‑1 (MQ=255)                                                                             aTCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGagagag                                                        <  1:366603/140‑1 (MQ=255)                                                                                 aCGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAgctgc                                                    >  2:40703/1‑140 (MQ=255)                                                                                    cATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCa                                                 <  2:261167/140‑1 (MQ=255)                                                                                     aTGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCAc                                                <  2:245868/140‑1 (MQ=255)                                                                                                   tCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTg                                  >  1:6177/1‑140 (MQ=255)                                                                                                      tGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTg                               >  1:160031/1‑140 (MQ=255)                                                                                                             gtCATTGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGa                        >  1:24768/1‑140 (MQ=255)                                                                                                                 ttGGCGTCGCAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGgt                    <  2:298527/140‑1 (MQ=255)                                                                                                                          cAGCCGGTCCAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTg           >  2:134405/1‑140 (MQ=255)                                                                                                                                   cAGGTGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGagcag  <  2:6177/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAG  >  NC_000913/830050‑830319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑