Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	840,470	G→A	S354S (AGC→AGT)	fiu ←	putative iron siderophore outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	840,470	0	G	A	100.0%	94.8 / NA	28	S354S (AGC→AGT)	fiu	putative iron siderophore outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

CGCCGGTACTGACATCATGACCGATAGAACCGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCGCTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACGGGTAGTATTGCGAATGGTGGTGTTATCGTTGATGTCGTGCTCAAAACGC  >  NC_000913/840340‑840596                                                                                                                                   |                                                                                                                               cGCCGGTACTGACATCATGACCGATAGAACCGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGc                                                                                                                       >  2:160356/1‑140 (MQ=255)  gCCGGTACTGACATCATGACCGATAGAACCGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCt                                                                                                                      <  1:288694/140‑1 (MQ=255)      gTACTGACATCATGACCGATAGAACCGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGt                                                                                                                  >  2:229680/1‑140 (MQ=255)      gTACTGACATCATGACCGATAGAACCGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGt                                                                                                                  >  2:189008/1‑140 (MQ=255)               tcatGACCGATAGAACCGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGa                                                                                                         <  1:20469/140‑1 (MQ=255)                 atGACCGATAGAACCGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGAcg                                                                                                       >  1:129411/1‑140 (MQ=255)                    aCCGATAGAACCGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGAcgccc                                                                                                    <  2:129411/140‑1 (MQ=255)                        aTAGAACCGGTATAGAACGTCGAGGTCAGGTTGGACTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGAcgccccgcc                                                                                                >  1:263454/1‑140 (MQ=255)                              ccGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAAt                                                                                          >  1:165408/1‑140 (MQ=255)                                ggTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGCGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCg                                                                                        <  1:157597/140‑1 (MQ=255)                                      gAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGtca                                                                                  <  1:160356/140‑1 (MQ=255)                                       aaCGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGtcat                                                                                 >  2:52557/1‑140 (MQ=255)                                           tCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAgg                                                                             <  1:292859/140‑1 (MQ=255)                                            cGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGt                                                                            >  1:371056/1‑140 (MQ=255)                                            cGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGt                                                                            >  1:302119/1‑140 (MQ=255)                                                gTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATc                                                                        <  1:52557/140‑1 (MQ=255)                                                  cAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCt                                                                      >  1:298463/1‑140 (MQ=255)                                                                  gtGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCa                                                      <  2:302119/140‑1 (MQ=255)                                                                    gagaATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAAc                                                    <  1:229680/140‑1 (MQ=255)                                                                       aaTTTTATTACTCACATCTTTGGTATTCGCCGTGTGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACggg                                                 <  2:7613/140‑1 (MQ=255)                                                                       aaTTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACggg                                                 <  2:165408/140‑1 (MQ=255)                                                                       aaTTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACggg                                                 <  1:238183/140‑1 (MQ=255)                                                                       aaTTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACggg                                                 <  2:298463/140‑1 (MQ=255)                                                                                    acaTCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACGGGTAGTATTGCGAAt                                    <  2:263454/140‑1 (MQ=255)                                                                                                  cGCCGTGCGTGACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACGGGTAGTATTGCGAATGGTGGTGTTATCGt                      >  2:366649/1‑140 (MQ=255)                                                                                                       tgcgtgACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACGGGTAGTATTGCGAATGGTGGTGTTATCGTTGATg                 >  1:390070/1‑140 (MQ=255)                                                                                                        gcgtgACCAGGTCCAGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACGGGTAGTATTGCGAATGGTGGTGTTATCGTTGATGt                >  2:387610/1‑140 (MQ=255)                                                                                                                      aGCTATTCACATCACTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACGGGTAGTATTGCGAATGGTGGTGTTATCGTTGATGTCGTGCTCAAAACGc  >  1:155971/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                               CGCCGGTACTGACATCATGACCGATAGAACCGGTATAGAACGTCGAGGTCAGGTTGGTCTGGTTGGTGAGAATTTTATTACTCACATCTTTGGTATTCGCCGTGCGTGACCAGGTCCAGCTATTCACATCGCTGGTGGGCTGAGTAATATTCGACGCCCCGCCCATAATCGCCGTCATCAGGTAATCCTGCTTTACGCGCGACCAACGGGTAGTATTGCGAATGGTGGTGTTATCGTTGATGTCGTGCTCAAAACGC  >  NC_000913/840340‑840596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑