Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	902,735	A→G	M85T (ATG→ACG)	artI ←	putative ABC transporter periplasmic binding protein ArtI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	902,735	0	A	G	100.0%	80.0 / NA	23	M85T (ATG→ACG)	artI	putative ABC transporter periplasmic binding protein ArtI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

GCCGACTTTTTTGCCTTTCAGCTGATCAACACTGGTGTATTTGCCTTGCTGACCCACAAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGCCAGGTCGACGTCAAAACCAACGAT  >  NC_000913/902599‑902862                                                                                                                                         |                                                                                                                                gCCGACTTTTTTGCCTTTCAGCTGATCAACACTGGTGTATTTGCCTTGCTGACCCACAAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCa                                                                                                                              <  2:367084/140‑1 (MQ=255)   cGACTTTTTTGCCTTTCAGCTGATCAACACTGGTGTATTTGCCTTGCTGACCCACAAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACg                                                                                                                            >  1:389931/1‑140 (MQ=255)     aCTTTTTTGCCTTTCAGCTGATCAACACTGGTGTATTTGCCTTGCTGACCCACAAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGc                                                                                                                          >  2:46573/1‑140 (MQ=255)           ttGCCTTTCAGCTGATCAACACTGGTGTATTTGCCTTGCTGACCCACAAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTAc                                                                                                                    >  2:334527/1‑140 (MQ=255)                            aaCACTGGTGTATTTGCCTTGCTGACCCACAAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGc                                                                                                   <  2:389931/140‑1 (MQ=255)                                   gtgtATTTGCCTTGCTGACCCACAAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGTAt                                                                                            >  2:360212/1‑140 (MQ=255)                                           gCCTTGCTGACCCACAAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGt                                                                                    >  2:67327/1‑140 (MQ=255)                                                         caAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTa                                                                      >  1:134811/1‑140 (MQ=255)                                                                  cAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTg                                                             <  1:161292/140‑1 (MQ=255)                                                                          tCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGc                                                     >  2:154790/1‑140 (MQ=255)                                                                               gTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCaa                                                <  1:67327/140‑1 (MQ=255)                                                                               gTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCaa                                                >  2:125061/1‑140 (MQ=255)                                                                               gTACGCGGTGGTAAACAGCACCTGCTTTTCACCCTCGGGACTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTCTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCaa                                                <  2:349912/140‑1 (MQ=255)                                                                                    ggtgggcaaaCAGCCCCGGCTTTTCCCGCTCCGGTGTATTATCCACGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATctct                                           <  2:289731/133‑1 (MQ=255)                                                                                    gggTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATctct                                           >  2:228476/1‑140 (MQ=255)                                                                                     ggtggtAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCtt                                          <  1:218747/140‑1 (MQ=255)                                                                                       tggtAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTa                                        >  1:349912/1‑140 (MQ=255)                                                                                                     tGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGTATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGc                          <  1:360212/140‑1 (MQ=255)                                                                                                      gCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGcc                         <  1:125061/140‑1 (MQ=255)                                                                                                                           tatCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGCCAGGTCGACGTCAAAACCAACg    <  2:238520/140‑1 (MQ=255)                                                                                                                            atCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGCCAGGTCGACGTCAAAACCAACGa   <  2:324994/140‑1 (MQ=255)                                                                                                                             tCCATGCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGCCAGGTCGACGTCAAAACCAACGAt  >  2:134667/1‑140 (MQ=255)                                                                                                                                  gCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGCCAGGTCGACGTCAAAACCAAc     >  2:289094/1‑132 (MQ=255)                                                                                                                                  gCCCGCCGTCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGCCAGGTCGACGTCAAAACCAAc     <  1:289094/132‑1 (MQ=255)                                                                                                                                         |                                                                                                                                GCCGACTTTTTTGCCTTTCAGCTGATCAACACTGGTGTATTTGCCTTGCTGACCCACAAACAGGGCAGAGTTGTCATAGTACGGGGTGGTAAACAGCACCTGCTTTTCACGCTCCGGAGTGATATCCATGCCCGCCATCACGGCTTCTACGCGACGGAATTTCAGGCTTGGGATCAGGCTGTCAAACGCCTGGTTAGAGAAAGTGCAGGTTGCATCAATCTCTTTACACAGCGCTTGTGCCAGGTCGACGTCAAAACCAACGAT  >  NC_000913/902599‑902862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑