Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	94,115	C→T	A317V (GCG→GTG)	murE →	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	94,115	0	C	T	100.0%	89.6 / NA	27	A317V (GCG→GTG)	murE	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/15);  total (12/15)

TGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCAT  >  NC_000913/93982‑94245                                                                                                                                      |                                                                                                                                   tGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTTATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACt                                                                                                                              >  1:140880/1‑140 (MQ=255)      gAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTgg                                                                                                                         >  1:252713/1‑140 (MQ=255)          gCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACt                                                                                                                     <  2:204878/140‑1 (MQ=255)                   gTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATcc                                                                                                            <  1:268847/140‑1 (MQ=255)                   gTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATcc                                                                                                            <  2:205542/140‑1 (MQ=255)                                    cGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGa                                                                                           >  1:307000/1‑140 (MQ=255)                                     ggTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATctg                                                                                               <  2:280792/135‑1 (MQ=255)                                     ggTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATctg                                                                                               >  1:280792/1‑135 (MQ=255)                                        gCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTg                                                                                                    >  1:83485/1‑127 (MQ=255)                                        gCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTg                                                                                                    <  2:83485/127‑1 (MQ=255)                                         cgacgaTTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAAcc                                                                                      >  1:55145/1‑140 (MQ=255)                                                           cAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCg                                                                    <  1:51305/140‑1 (MQ=255)                                                              gTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGtt                                                                 >  2:154261/1‑140 (MQ=255)                                                                  gggCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTACCGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCg                                                             >  2:27839/1‑140 (MQ=255)                                                                  gggCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCg                                                             <  1:261299/140‑1 (MQ=255)                                                                   ggCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCgg                                                            <  2:222946/140‑1 (MQ=255)                                                                   ggCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCgg                                                            <  2:252713/140‑1 (MQ=255)                                                                   ggCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCgg                                                            <  1:338474/140‑1 (MQ=255)                                                                        tGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTa                                                       >  2:72599/1‑140 (MQ=255)                                                                                         aTCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTgcg                                      <  1:317078/140‑1 (MQ=255)                                                                                          tCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTgcgc                                     >  2:79190/1‑140 (MQ=255)                                                                                                    gcTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAAcc                           <  1:79190/140‑1 (MQ=255)                                                                                                    gcTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAAcc                           <  2:307000/140‑1 (MQ=255)                                                                                                         taccGTCAGCAACCTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACgg                      <  1:27839/137‑1 (MQ=255)                                                                                                                     ccTGCTGCTCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGAtt          >  2:294469/1‑140 (MQ=255)                                                                                                                            ctCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCa   <  1:154261/140‑1 (MQ=255)                                                                                                                             tCGCGCTGGTGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCAt  >  2:128033/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                   TGGTTGAAAGCGACCGAAGTGAACTATCACGACAGCGGTGCGACGATTCGCTTTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCAT  >  NC_000913/93982‑94245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑