Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	953,662	A→G	L269L (TTG→CTG)	focA ←	formate channel FocA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	953,662	0	A	G	100.0%	58.3 / NA	17	L269L (TTG→CTG)	focA	formate channel FocA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/8);  total (9/8)

GGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGG  >  NC_000913/953528‑953798                                                                                                                                       |                                                                                                                                         ggCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAac                                                                                                                                     >  2:17485/1‑140 (MQ=255)                                 acctacctTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGa                                                                                                     <  2:124721/140‑1 (MQ=255)                                   ctacctTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAAt                                                                                                   <  1:17485/140‑1 (MQ=255)                                   ctacctTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAAt                                                                                                   <  2:231102/140‑1 (MQ=255)                                                 ggATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGtga                                                                                     >  1:184981/1‑140 (MQ=255)                                                   aTTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGtgatg                                                                                   <  2:184981/140‑1 (MQ=255)                                                                 gCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATtcat                                                                           <  1:125732/134‑1 (MQ=255)                                                                 gCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATtcat                                                                           >  2:125732/1‑134 (MQ=255)                                                                         cGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTgag                                                             >  1:183964/1‑140 (MQ=255)                                                                              aCCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGaaaaa                                                        >  2:46014/1‑140 (MQ=255)                                                                                cATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAAtt                                                      >  2:378713/1‑140 (MQ=255)                                                                                   tAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTc                                                   <  1:378713/140‑1 (MQ=255)                                                                                   tAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTc                                                   <  2:183964/140‑1 (MQ=255)                                                                                            tCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTc                                                   >  2:139507/1‑131 (MQ=255)                                                                                            tCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTc                                                   <  1:139507/131‑1 (MQ=255)                                                                                                          gTAAATGACCCAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTcc                            >  1:210583/1‑140 (MQ=255)                                                                                                                    cAGTATGTCAACCCAACCAGCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCgg                  >  1:361575/1‑140 (MQ=255)                                                                                                                                ccaaccagCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTc      >  1:266754/1‑140 (MQ=255)                                                                                                                                ccaaccagCAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTc      >  1:211219/1‑140 (MQ=255)                                                                                                                                 caaccagcAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTcg     >  2:209329/1‑140 (MQ=255)                                                                                                                                   accagcAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTcgcg   <  2:210583/140‑1 (MQ=255)                                                                                                                                    ccagcAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCgg  <  2:211219/140‑1 (MQ=255)                                                                                                                                    ccagcAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCgg  <  2:266754/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGG  >  NC_000913/953528‑953798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑