Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,062,967	A→G	V270A (GTA→GCA)	cbpA ←	curved DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,062,967	0	A	G	100.0%	38.6 / NA	12	V270A (GTA→GCA)	cbpA	curved DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/5);  total (7/5)

GCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGT  >  NC_000913/1062851‑1063091                                                                                                                     |                                                                                                                             gCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGt                                                                                                       >  1:349111/1‑140 (MQ=255)               ccccAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAg                                                                                         >  1:74014/1‑140 (MQ=255)                               gATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAAcg                                                                         >  1:168418/1‑140 (MQ=255)                                 tCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAAcgcg                                                                       <  2:267478/140‑1 (MQ=255)                                          gacTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTg                                                              >  1:235413/1‑140 (MQ=255)                                          gacTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTg                                                              >  2:379652/1‑140 (MQ=255)                                                                gcCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCggg                                        <  2:168418/140‑1 (MQ=255)                                                                       cgcgGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCa                                 >  1:318485/1‑140 (MQ=255)                                                                                       tCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGctttcttt                 <  2:318485/140‑1 (MQ=255)                                                                                       tCCGGTTTCGGCGGCATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGctttcttt                 <  2:74014/140‑1 (MQ=255)                                                                                                 gcggcATCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGa       >  1:60639/1‑140 (MQ=255)                                                                                                      aTCACGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt  <  2:112546/140‑1 (MQ=255)                                                                                                                     |                                                                                                                             GCCATTATGCTTTCCCCCAATCTTTACGTGGATCAAAAGACGACTGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGT  >  NC_000913/1062851‑1063091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑