Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,215,783	C→T	intergenic (‑308/‑6)	bluF ← / → ycgZ	blue light‑responsive regulator of BluR/putative two‑component system connector protein YcgZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,215,783	0	C	T	100.0%	78.1 / NA	23	intergenic (‑308/‑6)	bluF/ycgZ	blue light‑responsive regulator of BluR/putative two‑component system connector protein YcgZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/10);  total (13/10)

TATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGA  >  NC_000913/1215647‑1215907                                                                                                                                         |                                                                                                                               tatGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTa                                                                                                                             <  2:63300/140‑1 (MQ=255)                     tGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTg                                                                                                       >  1:61288/1‑140 (MQ=255)                                 aTTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCt                                                                                           >  2:266440/1‑140 (MQ=255)                                   tAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGc                                                                                         <  1:266440/140‑1 (MQ=255)                                      cACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCggg                                                                                      >  1:240629/1‑140 (MQ=255)                                           aTTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCaa                                                                                 >  2:116537/1‑140 (MQ=255)                                                                  tCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCt                                                          <  1:222106/140‑1 (MQ=255)                                                                           atatTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCAt                                                 >  1:70678/1‑140 (MQ=255)                                                                           atatTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCAt                                                 >  2:179855/1‑140 (MQ=255)                                                                            tatTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATa                                                <  1:366744/140‑1 (MQ=255)                                                                                 cGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTcag                                           <  1:179855/140‑1 (MQ=255)                                                                                     cAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCCTACTCAGCAgg                                       >  2:303904/1‑140 (MQ=255)                                                                                           tGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAActc                                 >  1:73621/1‑140 (MQ=255)                                                                                                  gTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAGCACATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCggg                            >  1:7599/1‑140 (MQ=255)                                                                                                  gTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGa                          >  2:131597/1‑140 (MQ=255)                                                                                                              aaCATTGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTga              <  1:116537/140‑1 (MQ=255)                                                                                                                   tGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACt                >  1:28665/1‑133 (MQ=255)                                                                                                                   tGATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACt                <  2:28665/133‑1 (MQ=255)                                                                                                                    gATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTga              >  1:282860/1‑134 (MQ=255)                                                                                                                    gATTAACATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTga              <  2:282860/134‑1 (MQ=255)                                                                                                                          cATTTTTAACAGAGGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCCTACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGa  <  1:303904/140‑1 (MQ=255)                                                                                                                                     gagGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGa  <  1:294093/129‑1 (MQ=255)                                                                                                                                     gagGTGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGa  >  2:294093/1‑129 (MQ=255)                                                                                                                                         |                                                                                                                               TATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAG‑‑CATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATACTCAGCAGGAAACTCTCGGGGAAATTGTGACTGAGATTTTGAAAGA  >  NC_000913/1215647‑1215907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑