Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,246,801	A→G	G192G (GGT→GGC)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,246,801	0	A	G	100.0%	38.0 / NA	12	G192G (GGT→GGC)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/8);  total (4/8)

ACCATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGACCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCAAGTCCTAACATGGTGAAGTAACTGTCCCA  >  NC_000913/1246733‑1246902                                                                     |                                                                                                       aCCATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCgg                                 <  1:297818/139‑1 (MQ=255) aCCATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCgg                                 >  2:297818/1‑139 (MQ=255)  ccATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCa                               <  2:39201/140‑1 (MQ=255)     tCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCAAGt                            >  1:306101/1‑140 (MQ=255)       aGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCgg                                 <  1:209348/133‑1 (MQ=255)       aGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCgg                                 >  2:209348/1‑133 (MQ=255)             aagaagGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCAAGTCCTAACAt                    <  2:73639/140‑1 (MQ=255)                       gTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCAAGTCCTAACATGGTGAAGTaa          <  2:306101/140‑1 (MQ=255)                          gCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCAAGTCCTAACATGGTGAAGTAACTg       >  2:155614/1‑140 (MQ=255)                            gAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTTCCATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCAAGTCCTAACATGGTGAAGTAACTGt      <  2:137110/140‑1 (MQ=255)                               cGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCAAGTCCTAACATGGTGAAGTAACTGTCCCa  <  1:155614/140‑1 (MQ=255)                               cGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGGCCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCAAGTCCTAACATGGTGAAGTAACTGTCCCa  <  1:395919/140‑1 (MQ=255)                                                                     |                                                                                                       ACCATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAATATGACCGTAAGTGTCTATTT‑CATGAGCAAAATTGGCCACCATATCCGCGACTTTATCCCAGTGACCGCTTTCGGCAAGTCCTAACATGGTGAAGTAACTGTCCCA  >  NC_000913/1246733‑1246902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑