Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,480,925	G→A	G6D (GGC→GAC)	ynbD →	putative phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,480,925	0	G	A	100.0%	94.6 / NA	28	G6D (GGC→GAC)	ynbD	putative phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/16);  total (12/16)

ATGGATTCACTCGTGCGTGATGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCC  >  NC_000913/1480794‑1481060                                                                                                                                    |                                                                                                                                        aTGGATTCACTCGTGCGTGATGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTAt                                                                                                                                 >  2:243707/1‑140 (MQ=255)             gtgcgtgATGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGccc                                                                                                                     <  2:157380/140‑1 (MQ=255)                cgtgATGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGt                                                                                                                  >  1:352089/1‑140 (MQ=255)                   gATGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGtttt                                                                                                               >  2:35925/1‑140 (MQ=255)                     tGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTttc                                                                                                             >  1:111973/1‑140 (MQ=255)                             ttGATAAATGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCAc                                                                                                     <  1:386612/140‑1 (MQ=255)                                  aaaTGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATg                                                                                                <  1:47520/140‑1 (MQ=255)                                   aaTGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATgg                                                                                               <  2:267989/140‑1 (MQ=255)                                   aaTGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATgg                                                                                               <  1:243707/140‑1 (MQ=255)                                   aaTGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATgg                                                                                               <  2:111973/140‑1 (MQ=255)                                       cacacaACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGAtct                                                                                           >  1:353188/1‑140 (MQ=255)                                            aaCGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATgg                                                                                               <  1:157692/131‑1 (MQ=255)                                            aaCGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATgg                                                                                               >  2:157692/1‑131 (MQ=255)                                                 aTTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGt                                                                                 >  2:343730/1‑140 (MQ=255)                                                      tgaGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCAcc                                                                            >  2:58342/1‑140 (MQ=255)                                                      tgaGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCAcc                                                                            >  2:294688/1‑140 (MQ=255)                                                       gaGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACcg                                                                           <  2:353188/140‑1 (MQ=255)                                                       gaGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACcg                                                                           <  1:343730/140‑1 (MQ=255)                                                       gaGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACcg                                                                           <  1:25520/140‑1 (MQ=255)                                                       gaGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACcg                                                                           <  2:318681/140‑1 (MQ=255)                                                            gggTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGtt                                                                      <  1:58342/140‑1 (MQ=255)                                                                            tCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGcc                                                      <  1:35925/140‑1 (MQ=255)                                                                            tCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGcc                                                      >  2:45213/1‑140 (MQ=255)                                                                                    ggTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATc                                              <  1:85220/140‑1 (MQ=255)                                                                                      tGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATccc                                            <  2:134206/140‑1 (MQ=255)                                                                                                                 gTATTGCTACAAGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGc                 <  1:294688/140‑1 (MQ=255)                                                                                                                            aGGCGCTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTttc      >  2:47452/1‑140 (MQ=255)                                                                                                                               cgcTGACTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTttcttc   >  1:140648/1‑140 (MQ=255)                                                                                                                                gctgactgGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTcc  <  1:47452/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        ATGGATTCACTCGTGCGTGATGCCGGATTTGATAAATGCACACAACGGATTGATGAGTGGGGTATTTTTACGGTTTCGATGGCGGTGCGTCGTGATAACTGAACGTCGGAACGTATTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCC  >  NC_000913/1480794‑1481060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑