Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	140,126	C→T	G367D (GGT→GAT)	gcd ←	quinoprotein glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	140,126	0	C	T	100.0%	74.5 / NA	22	G367D (GGT→GAT)	gcd	quinoprotein glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

GCGTCATAGGCCGCTGGTGCCCAGGAGTTTGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGG  >  NC_000913/139994‑140243                                                                                                                                     |                                                                                                                      gcgTCATAGGCCGCTGGTGCCCAGGAGTTTGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGAt                                                                                                                <  1:274623/140‑1 (MQ=255)               tGGTGCCCAGGAGTTTGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGttt                                                                                                  >  2:140102/1‑140 (MQ=255)                  tGCCCAGGAGTTTGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTcgc                                                                                               <  1:216209/140‑1 (MQ=255)                            tttGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGaa                                                                                     >  2:61385/1‑140 (MQ=255)                            tttGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGaa                                                                                     >  2:204821/1‑140 (MQ=255)                              tGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGt                                                                                   >  2:111854/1‑140 (MQ=255)                               ggCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGtt                                                                                  <  2:387132/140‑1 (MQ=255)                                               aggtGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCg                                                                  >  2:44860/1‑140 (MQ=255)                                                 gtgtgtTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGc                                                                <  2:318262/140‑1 (MQ=255)                                                    tgtTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCAt                                                             <  1:140102/140‑1 (MQ=255)                                                         gTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGa                                                        <  1:111854/140‑1 (MQ=255)                                                                   aTTGCGTTCGGATCTTTAGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAGTCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATc                                              >  1:345432/1‑140 (MQ=255)                                                                     tGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCgg                                            >  1:54239/1‑140 (MQ=255)                                                                        gatCGGAACTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGa                                         >  2:215026/3‑140 (MQ=255)                                                                            ggATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAAt                                     >  2:229033/1‑140 (MQ=255)                                                                                   tCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGc                              >  2:108283/1‑140 (MQ=255)                                                                                         cGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTc                        <  1:61385/140‑1 (MQ=255)                                                                                         cGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTc                        <  1:204821/140‑1 (MQ=255)                                                                                         cGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTc                        <  1:108283/140‑1 (MQ=255)                                                                                            gATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGt                     <  2:54239/140‑1 (MQ=255)                                                                                                    gCCCACAGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAg             <  2:110921/140‑1 (MQ=255)                                                                                                       cacaGCAGCTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGAcc          <  1:44860/140‑1 (MQ=255)                                                                                                               cTCCCCGGTGTTGACATCAAAATCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTgg  >  1:33087/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                      GCGTCATAGGCCGCTGGTGCCCAGGAGTTTGGCGAGTTAAAGGTAAAGGTGTGTTCGTCAGACGGGATTGCGTTCGGATCTTTCGCGCCGGGATCAAAAGCCCACAGCAGCTCCCCGGTGTTGACATCAAAACCACGGATCACGCCAGACGTTTCGCGGGTTGAGAAGTTATCGGTAACTGAACCGGCCATCACGATGGTTTTATCGGTGATAATCGGTGGCGAAGTCGGTTCATACAGACCCGGTTTGG  >  NC_000913/139994‑140243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑