Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,810,896	T→C	intergenic (+95/‑38)	ydjM → / → tcyP	putative inner membrane protein regulated by LexA/cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,810,896	0	T	C	100.0%	42.4 / NA	13	intergenic (+95/‑38)	ydjM/tcyP	putative inner membrane protein regulated by LexA/cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/6);  total (7/6)

ATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTC  >  NC_000913/1810760‑1811018                                                                                                                                         |                                                                                                                           aTCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACg                                                                                                                         <  1:88814/140‑1 (MQ=255) aTCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACg                                                                                                                         <  2:173839/140‑1 (MQ=255)           aTCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACGCAGTGATAGa                                                                                                               >  1:23108/1‑140 (MQ=255)                            cAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGgag                                                                                              >  2:30247/1‑140 (MQ=255)                                           aaaGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACtt                                                                               <  1:30247/140‑1 (MQ=255)                                                  aaaaTCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTa                                                                        >  2:228679/1‑140 (MQ=255)                                                                      aGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGtt                                                    <  2:240504/140‑1 (MQ=255)                                                                        cAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCg                                                  <  2:139994/140‑1 (MQ=255)                                                                                tttGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGtt                                                <  1:290646/134‑1 (MQ=255)                                                                                tttGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGtt                                                >  2:290646/1‑134 (MQ=255)                                                                                                aGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTc                          >  1:182710/1‑140 (MQ=255)                                                                                                                   cTTGCGCCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTg       >  1:189170/1‑140 (MQ=255)                                                                                                                        gcCAACACTATGACTGCCACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTc  >  1:212580/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                           ATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTTAATCTTGCGCCAACACTATGACTGCTACGCAGTGATAGAAATAATAAGATCAGGAGAACGGGGATGAACTTTCCATTAATTGCGAACATCGTGGTGTTCGTTGTACTGCTGTTTGCGCTGGCTCAGACCCGCCATAAACAGTGGAGTC  >  NC_000913/1810760‑1811018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑