Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,843,294	A→G	Q308Q (CAA→CAG)	gdhA →	glutamate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,843,294	0	A	G	100.0%	31.1 / NA	10	Q308Q (CAA→CAG)	gdhA	glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/7);  total (3/7)

GACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCGAAGGGGCAAATATGCCGAC  >  NC_000913/1843157‑1843422                                                                                                                                          |                                                                                                                                 gACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGGGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAGCa                                                                                                                                >  2:819/1‑140 (MQ=255)    tCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCATAGAATTTGGTCTGGTCTATCTCGAAGGCCAGCAGcc                                                                                                                             <  1:168091/140‑1 (MQ=255)     ccAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAGCAGCCg                                                                                                                            <  1:247322/140‑1 (MQ=255)       aGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAGCAGCCGTg                                                                                                                          <  2:84346/140‑1 (MQ=255)                                  ttCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAGCAGCCGTGGTCTCTACCGGTTGATATCGCCCTGcc                                                                                               <  2:168482/140‑1 (MQ=255)                                           gagaAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAGCAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCAc                                                                                      <  1:142030/140‑1 (MQ=255)                                                              tCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAGCAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGtt                                                                   >  1:298076/1‑140 (MQ=255)                                                                   aTCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAGCAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGAcgc                                                              <  2:61447/140‑1 (MQ=255)                                                                                                                        ggtctATCTCGAAGGCCAGCAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCGAAGGGGCAAata         >  2:381777/1‑140 (MQ=255)                                                                                                                              tctcGAAGGCCAGCAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCGAAGGGGCAAATATGCCGa   >  1:196352/1‑140 (MQ=255)                                                                                                                               ctcGAAGGCCAGCAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCGAAGGGGCAAATATGCCGac  <  2:298076/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 GACTCCAGCGGCACTGTAGTTGATGAAAGCGGATTCACGAAAGAGAAACTGGCACGTCTTATCGAAATCAAAGCCAGCCGCGATGGTCGAGTGGCAGATTACGCCAAAGAATTTGGTCTGGTCTATCTCGAAGGCCAACAGCCGTGGTCTCTACCGGTTGATATCGCCCTGCCTTGCGCCACCCAGAATGAACTGGATGTTGACGCCGCGCATCAGCTTATCGCTAATGGCGTTAAAGCCGTCGCCGAAGGGGCAAATATGCCGAC  >  NC_000913/1843157‑1843422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑