Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,910,536	T→C	G87G (GGT→GGC)	yebQ →	putative transporter YebQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,910,536	0	T	C	100.0%	53.0 / NA	16	G87G (GGT→GGC)	yebQ	putative transporter YebQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/8);  total (8/8)

CGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGC  >  NC_000913/1910421‑1910665                                                                                                                    |                                                                                                                                  cGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCa                                                                                                           <  2:344846/140‑1 (MQ=255)                tCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCtt                                                                                            >  2:84734/1‑140 (MQ=255)                                          gCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCt                                                                  <  1:276824/140‑1 (MQ=255)                                          gCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCt                                                                  >  2:239105/1‑140 (MQ=255)                                                           gCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAg                                                 >  1:171416/1‑140 (MQ=255)                                                                       gtttCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTg                                     >  2:109113/1‑140 (MQ=255)                                                                                  atatGTTTGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTtgatg                          <  2:141712/140‑1 (MQ=255)                                                                                   tatGTTTGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTtgatga                         >  2:116328/1‑140 (MQ=255)                                                                                       tttGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGt                     <  1:239105/140‑1 (MQ=255)                                                                                        ttGGCTATCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGtt                    >  1:131125/1‑140 (MQ=255)                                                                                               tCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCg             <  1:116328/140‑1 (MQ=255)                                                                                               tCGACGTATTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCg             >  2:82140/1‑140 (MQ=255)                                                                                                      aTTTATAAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTAt      >  2:372408/1‑140 (MQ=255)                                                                                                          ataAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGc  <  1:372408/140‑1 (MQ=255)                                                                                                          ataAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGc  <  1:82140/140‑1 (MQ=255)                                                                                                          ataAATGCGGCCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGc  <  2:67282/140‑1 (MQ=255)                                                                                                                    |                                                                                                                                  CGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGTCATACAAGGTTTCGGCGGTGCAGCGTTGATGAGCGTTAATACCGCACTTATCCGC  >  NC_000913/1910421‑1910665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑