Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,010,684	T→C	S29P (TCT→CCT)	yedL →	putative acetyltransferase YedL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,010,684	0	T	C	100.0%	87.2 / NA	25	S29P (TCT→CCT)	yedL	putative acetyltransferase YedL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/11);  total (14/11)

TCATTACCTGTTGGCATATTGCAAAAATAACACCAATACGGAATCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAA  >  NC_000913/2010552‑2010808                                                                                                                                     |                                                                                                                             tCATTACCTGTTGGCATATTGCAAAAATAACACCAATACGGAATCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTccccc                                                                                                                       >  1:103353/1‑140 (MQ=255) tCATTACCTGTTGGCATATTGCAAAAATAACACCAATACGGAATCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTccccc                                                                                                                       >  2:209727/1‑140 (MQ=255)     tACCTGTTGGCATATTGCAAAAATAACACCAATACGGAATCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGaa                                                                                                                   <  1:172430/140‑1 (MQ=255)           ttGGCATATTGCAAAAATAACACCAATACGGAATCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGt                                                                                                             <  1:209727/140‑1 (MQ=255)                     gCAAAAATAACACCAATACGGAATCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGt                                                                                                             >  1:215954/1‑130 (MQ=255)                     gCAAAAATAACACCAATACGGAATCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGt                                                                                                             <  2:215954/130‑1 (MQ=255)                                          aaTCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAAcc                                                                              >  2:248719/1‑140 (MQ=255)                                          aaTCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAAcc                                                                              <  1:299114/140‑1 (MQ=255)                                                                         ctcACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAgg                                               >  2:5445/1‑140 (MQ=255)                                                                                    agcagTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCg                                    >  1:381618/1‑140 (MQ=255)                                                                                        gTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAgg                                >  2:236388/1‑140 (MQ=255)                                                                                          gCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGaa                              >  2:287330/1‑140 (MQ=255)                                                                                          gCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGATCAGTATGGGAAGGCGAACAACTCGCAGGaa                              >  1:32939/1‑140 (MQ=255)                                                                                           cAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAAt                             >  2:229436/1‑140 (MQ=255)                                                                                             aGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTg                           <  1:229436/140‑1 (MQ=255)                                                                                                   aGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTgcgc                     >  2:112622/1‑140 (MQ=255)                                                                                                   aGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTgc                       >  1:328748/1‑138 (MQ=255)                                                                                                   aGTGGCTTACCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTgc                       <  2:328748/138‑1 (MQ=255)                                                                                                           aCCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAAt                             >  1:293869/1‑124 (MQ=255)                                                                                                           aCCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAAt                             <  2:293869/124‑1 (MQ=255)                                                                                                           aCCATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTg             <  1:287330/140‑1 (MQ=255)                                                                                                             cATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCt           <  1:5445/140‑1 (MQ=255)                                                                                                             cATATTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGATCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCt           <  2:32939/140‑1 (MQ=255)                                                                                                              atatTTCCGGCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTg          <  2:103353/140‑1 (MQ=255)                                                                                                                      ggCATGCTGCAGCAGCCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATaa  >  2:209350/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                             TCATTACCTGTTGGCATATTGCAAAAATAACACCAATACGGAATCGTCATGTTCACGATTAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAA  >  NC_000913/2010552‑2010808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑