Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,526	T→C	T151A (ACC→GCC)	rfbD ←	dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,526	0	T	C	100.0%	77.7 / NA	22	T151A (ACC→GCC)	rfbD	dTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/13);  total (9/13)

TACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGT  >  NC_000913/2111391‑2111660                                                                                                                                        |                                                                                                                                       tACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCgg                                                                                                                                    >  2:44792/1‑140 (MQ=255)  aCAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGa                                                                                                                                   >  1:82808/1‑140 (MQ=255)      tCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTTATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGATATTTCCGTTTCCTGCATAGACCCAGCTGGCCCGGAAAAt                                                                                                                               >  2:349754/1‑140 (MQ=255)      tCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATCCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAAt                                                                                                                               >  2:247601/1‑140 (MQ=255)         gccagcAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAg                                                                                                                            <  2:82808/140‑1 (MQ=255)          ccagcAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGa                                                                                                                           <  1:316539/140‑1 (MQ=255)                 ttCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTcg                                                                                                                    <  2:236175/140‑1 (MQ=255)                        ccTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTcgcg                                                                                                                  >  2:171803/1‑135 (MQ=255)                        ccTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTcgcg                                                                                                                  <  1:171803/135‑1 (MQ=255)                                    ccAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGc                                                                                                 >  2:314637/1‑140 (MQ=255)                                                         gCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGt                                                                            <  1:48842/140‑1 (MQ=255)                                                          cTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGtt                                                                           <  1:30622/140‑1 (MQ=255)                                                                   cACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTaa                                                                  <  1:54505/140‑1 (MQ=255)                                                                                         cATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCAt                                            <  2:176368/140‑1 (MQ=255)                                                                                               tttGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGtct                                      <  2:339150/140‑1 (MQ=255)                                                                                                ttGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGtctc                                     <  1:154440/140‑1 (MQ=255)                                                                                                            tttccttttcctGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCat                         <  2:19614/140‑1 (MQ=255)                                                                                                                  tttcctGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGcc                   <  1:44792/140‑1 (MQ=255)                                                                                                                  tttcctGCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGcc                   >  2:116994/1‑140 (MQ=255)                                                                                                                        gCATAGACCCAGCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTcc             >  2:147841/1‑140 (MQ=255)                                                                                                                                   gCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGt  <  1:314637/140‑1 (MQ=255)                                                                                                                                   gCTGGCCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGt  >  1:161022/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCTGCATAGACCCAGCTGGTCCGGAAAATAAGATGCTTCGCGCAATATTCCTGTAACGCTTTTTCTCCGGCTAACTTGGTTTCACCGTAAACATTTAGTGGTGCGGTTGCATCCGTCTCCAGCCATGGCATATCGCCATTTCCAGGGAAGACGT  >  NC_000913/2111391‑2111660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑