Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,139,283	T→C	V461A (GTG→GCG)	yegH →	inner membrane protein YegH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,139,283	0	T	C	100.0%	73.2 / NA	21	V461A (GTG→GCG)	yegH	inner membrane protein YegH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/10);  total (11/10)

ACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAG  >  NC_000913/2139148‑2139418                                                                                                                                        |                                                                                                                                        aCGCCACTAAACCCATTGCCGATAACTTACCGAACGAAGGGGAAGAGACCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGcgccg                                                                                                                                     <  2:10130/140‑1 (MQ=255)       cTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGcgccgctgccg                                                                                                                               <  1:301501/140‑1 (MQ=255)               aTTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGa                                                                                                                       >  2:174123/1‑140 (MQ=255)                ttGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGaa                                                                                                                      >  2:157007/1‑140 (MQ=255)                 tGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGaaa                                                                                                                     >  1:292556/1‑140 (MQ=255)                      gTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGt                                                                                                                <  2:237203/140‑1 (MQ=255)                                 aaggaggTGGAAGAGATCGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCacac                                                                                                     <  2:292651/134‑1 (MQ=255)                                 aacgaaGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCacac                                                                                                     <  1:174123/140‑1 (MQ=255)                                          gAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCgg                                                                                            <  2:292556/140‑1 (MQ=255)                                                  cgccgcccgccATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGa                                                                                    <  2:290493/137‑1 (MQ=255)                                                                           aaTGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCaaa                                                           >  1:37810/1‑140 (MQ=255)                                                                             tGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAAc                                                         >  2:67337/1‑140 (MQ=255)                                                                                    ggTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCga                                                  <  1:114746/140‑1 (MQ=255)                                                                                     gTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCgaa                                                 <  2:37810/140‑1 (MQ=255)                                                                                                gCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGt                                      >  2:230850/1‑140 (MQ=255)                                                                                                gCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGt                                      >  2:47660/1‑140 (MQ=255)                                                                                                                cGCTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCtt                      >  2:332135/1‑140 (MQ=255)                                                                                                                  cTGGAGGATCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTaa                    >  2:343202/1‑140 (MQ=255)                                                                                                                      aggaTCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACg                <  1:67337/140‑1 (MQ=255)                                                                                                                      aggaTCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACg                >  1:360520/1‑140 (MQ=255)                                                                                                                         aTCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTg             <  1:332135/140‑1 (MQ=255)                                                                                                                         aTCTGGTGCAATATGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTg             <  1:157007/140‑1 (MQ=255)                                                                                                                                    tatGCGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAg  >  1:195216/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAG  >  NC_000913/2139148‑2139418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑