Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,191,241	A→G	G135G (GGT→GGC)	yehC ←	putative fimbrial chaperone YehC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,191,241	0	A	G	100.0%	73.0 / NA	21	G135G (GGT→GGC)	yehC	putative fimbrial chaperone YehC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

TCATTTTTTATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGC  >  NC_000913/2191104‑2191346                                                                                                                                          |                                                                                                          tCATTTTTTATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGcc                                                                                                         >  2:90368/1‑140 (MQ=255)    ttttttATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCcttc                                                                                                      <  1:335701/140‑1 (MQ=255)       tttATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTg                                                                                                   <  1:312046/140‑1 (MQ=255)         tATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGct                                                                                                 <  2:307534/140‑1 (MQ=255)           tCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGctct                                                                                               >  2:156009/1‑140 (MQ=255)                     aTTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTtg                                                                                     <  2:69331/140‑1 (MQ=255)                       tGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTtg                                                                                     <  1:35380/138‑1 (MQ=255)                       tGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTtg                                                                                     >  2:35380/1‑138 (MQ=255)                          cACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGa                                                                                <  1:90368/140‑1 (MQ=255)                                         gCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTc                                                                 >  1:39417/1‑140 (MQ=255)                                                     aTGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATg                                                     <  1:63469/140‑1 (MQ=255)                                                       gTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGct                                                   <  1:156009/140‑1 (MQ=255)                                                       gTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGct                                                   <  2:39417/140‑1 (MQ=255)                                                       gTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGct                                                   <  2:239287/140‑1 (MQ=255)                                                         cGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGcttt                                                 <  1:106560/140‑1 (MQ=255)                                                                   aCCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGt                                       >  2:49589/1‑140 (MQ=255)                                                                     cGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTgg                                     <  2:365214/140‑1 (MQ=255)                                                                                  gCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCgg                        >  2:99764/1‑140 (MQ=255)                                                                                       ccGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATaa                   >  2:277481/1‑140 (MQ=255)                                                                                          gTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAAttt                >  2:386683/1‑140 (MQ=255)                                                                                                        ttCTGTTTTGCATCGCAAACTTCAGTGCATTCTTGCCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTtgc  >  2:133552/1‑140 (MQ=255)                                                                                                                                          |                                                                                                          TCATTTTTTATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGC  >  NC_000913/2191104‑2191346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑