Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,224,977	A→G	intergenic (‑107/+67)	pbpG ← / ← yohC	peptidoglycan DD‑endopeptidase PbpG/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,224,977	0	A	G	100.0%	54.2 / NA	16	intergenic (‑107/+67)	pbpG/yohC	peptidoglycan DD‑endopeptidase PbpG/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/8);  total (8/8)

AAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACT  >  NC_000913/2224849‑2225098                                                                                                                                 |                                                                                                                          aaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGt                                                                                                                <  1:26856/140‑1 (MQ=255)  aGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTg                                                                                                               >  1:332192/1‑140 (MQ=255)                    cATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCt                                                                                             <  2:333744/140‑1 (MQ=255)                      tgatgaGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGa                                                                                           >  2:50294/1‑140 (MQ=255)                            gCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTa                                                                                     >  2:355896/1‑140 (MQ=255)                                                                ccgccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACa                                                 <  1:13248/140‑1 (MQ=255)                                                                ccgccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACa                                                 >  2:128569/1‑140 (MQ=255)                                                                                   cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                              >  1:136600/1‑140 (MQ=255)                                                                                   cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                              <  1:216078/140‑1 (MQ=255)                                                                                         cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                        <  1:50294/140‑1 (MQ=255)                                                                                         cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                        <  2:136600/140‑1 (MQ=255)                                                                                                  ccATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                              >  1:150800/1‑125 (MQ=255)                                                                                                  ccATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                              <  2:150800/125‑1 (MQ=255)                                                                                                  ccATCATAACGAGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAg               >  1:204893/1‑140 (MQ=255)                                                                                                             aGCAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAgcagca    >  1:352760/1‑140 (MQ=255)                                                                                                               cAAAAAGTGCGATCGTCAGAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACt  <  1:162503/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                          AAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACT  >  NC_000913/2224849‑2225098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑