Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,256,899	G→A	A146V (GCG→GTG)	psuT ←	putative pseudouridine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,256,899	0	G	A	100.0%	80.1 / NA	24	A146V (GCG→GTG)	psuT	putative pseudouridine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/12);  total (12/12)

CCAATCATCATCGAACCAGCAATGGACGCCATCCCGCTACAAATTGCGGTAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCGCAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATGTAGTACAGCAGACTGATGAGCGCAGTAACGAAAATAATCGCCGGA  >  NC_000913/2256764‑2257030                                                                                                                                        |                                                                                                                                    ccAATCATCATCGAACCAGCAATGGACGCCATCCCGCTACAAATTGCGGTAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCAcaac                                                                                                                                 >  2:169379/1‑140 (MQ=255)           tcGAACCAGCAATGGACGCCATCCCGCTACAAATTGCGGTAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCg                                                                                                                       >  1:35844/1‑140 (MQ=255)             gAACCAGCAATGGACGCCATCCCGCTACAAATTGCGGTAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGAt                                                                                                                     >  1:96187/1‑140 (MQ=255)                 cAGCAATGGACGCCATCCCGCTACAAATTGCGGTAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTg                                                                                                                 >  2:120661/1‑140 (MQ=255)                             ccATCCCGCTACAAATTGCGGTAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAgg                                                                                                     <  1:343493/140‑1 (MQ=255)                                  ccGCTACAAATTGCGGTAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCttt                                                                                                <  1:208801/140‑1 (MQ=255)                                     cTACAAATTGCGGTAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTg                                                                                             >  1:32795/1‑140 (MQ=255)                                                  tAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCa                                                                                >  2:371621/1‑140 (MQ=255)                                                              tGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATc                                                                    >  2:191388/1‑140 (MQ=255)                                                                         cgatcgatAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCAt                                                         <  2:96187/140‑1 (MQ=255)                                                                         cgatcgatAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCAt                                                         <  1:120661/140‑1 (MQ=255)                                                                              gatAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGc                                                    <  2:35844/140‑1 (MQ=255)                                                                              gatAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGc                                                    <  1:371621/140‑1 (MQ=255)                                                                                   aCGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATg                                               <  2:250102/140‑1 (MQ=255)                                                                                      gTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGTCAAGGATGCGAATCAGCAGCCCCATCACGCCAATgtag                                            >  1:179249/1‑140 (MQ=255)                                                                                          aaCGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATgtagta                                          <  1:26701/138‑1 (MQ=255)                                                                                          aaCGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATgtagta                                          >  2:26701/1‑138 (MQ=255)                                                                                                 gCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATgtagt                                           <  2:43343/130‑1 (MQ=255)                                                                                                 gCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATgtagt                                           >  1:43343/1‑130 (MQ=255)                                                                                                    gggATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATGTAGTACAGCAGACtgat                              <  1:384060/140‑1 (MQ=255)                                                                                                    gggATCTCATTTTGCCCGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATGTAGTACAGCAGACtgat                              <  1:169379/140‑1 (MQ=255)                                                                                                                  cccGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATGTAGTACAGCAGACTGATGAGCGCAGTAACGa                >  2:277979/1‑140 (MQ=255)                                                                                                                  cccGAGGAAAATAGTAGTAACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATGTAGTACAGCAGACTGATGAGCGCAGTAACGa                >  1:370946/1‑140 (MQ=255)                                                                                                                                tagtaACCACAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATGTAGTACAGCAGACTGATGAGCGCAGTAACGAAAATAATCGCCGGa  <  1:191388/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CCAATCATCATCGAACCAGCAATGGACGCCATCCCGCTACAAATTGCGGTAAACAACTCGTTGCGATTCATGCGATCGATAAACGGTTTAACGATCGCCGGGATCTCATTTTGCCCGAGGAAAATAGTAGTAACCGCAACAAAAGATTCGATTTTGCTGATGTTGAGGGCTTTCTGGAAAATGCTGCCAAGGATGCGAATCAGCAGCCCCATCACGCCAATGTAGTACAGCAGACTGATGAGCGCAGTAACGAAAATAATCGCCGGA  >  NC_000913/2256764‑2257030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑