Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,337,269	T→C	T718A (ACC→GCC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,337,269	0	T	C	100.0%	70.5 / NA	21	T718A (ACC→GCC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

TATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCTTCAGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCGTC  >  NC_000913/2337139‑2337389                                                                                                                                   |                                                                                                                         tATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGcc                                                                                                                 >  1:307945/1‑140 (MQ=255)  aTTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGccc                                                                                                                >  1:391004/1‑140 (MQ=255)  aTTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGccc                                                                                                                <  1:116768/140‑1 (MQ=255)   ttCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCa                                                                                                               >  2:166224/1‑140 (MQ=255)    tCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCAt                                                                                                              <  1:288409/140‑1 (MQ=255)         cACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGc                                                                                                                     >  2:92241/1‑128 (MQ=255)         cACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGc                                                                                                                     <  1:92241/128‑1 (MQ=255)             gCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGAc                                                                                                     >  2:7775/1‑140 (MQ=255)                  aCGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACagaag                                                                                                <  1:166224/140‑1 (MQ=255)                   cGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACagaaga                                                                                               <  1:7775/140‑1 (MQ=255)                   cGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACagaaga                                                                                               <  2:325187/140‑1 (MQ=255)                           gTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTaa                                                                                       >  1:322928/1‑140 (MQ=255)                           gTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTaa                                                                                       >  1:159859/1‑140 (MQ=255)                               ccGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAgcg                                                                                   <  2:341582/140‑1 (MQ=255)                                 gTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGca                                                                                 <  2:391004/140‑1 (MQ=255)                                                                                  gagaCGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCTTCAGCGGAGAACAGCATTACTTCGTCTTCGCCGCTggt                                <  1:310983/140‑1 (MQ=255)                                                                                       gacTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCTTCAGCGGAGAACAGCATTACTTCGTCTTCGCCGCTggtcaggt                           >  1:63527/1‑140 (MQ=255)                                                                                       gacTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCTTCAGCGGAGAACAGCATTACTTCGTCTTCGCCGCTggtcaggt                           <  2:159859/140‑1 (MQ=255)                                                                                            tATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCTTCAGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACg                      >  2:127654/1‑140 (MQ=255)                                                                                                                aTACCGCGAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCTTCAGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCGTc  >  1:136397/1‑140 (MQ=255)                                                                                                                      cgAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCTTCAGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGcc     >  2:190939/1‑131 (MQ=255)                                                                                                                      cgAACACCGGTGGCGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCTTCAGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGcc     <  1:190939/131‑1 (MQ=255)                                                                                                                                   |                                                                                                                         TATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCTTCAGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCGTC  >  NC_000913/2337139‑2337389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑