| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,454,607 | A→T | pseudogene (395/2646 nt) | yfcU ← | putative outer membrane usher protein YfcU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,454,607 | 0 | A | T | 84.6% | 32.2 / 0.8 | 13 | pseudogene (395/2646 nt) | yfcU | putative outer membrane usher protein YfcU |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (6/5); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
CGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTT > NC_000913/2454480‑2454742 | cGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCTCg > 1:326619/1‑130 (MQ=255) cAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCCGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCTCGCCTTCCAGTTGa > 1:30796/1‑140 (MQ=255) gaTGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGc > 2:46185/1‑140 (MQ=255) cGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCt < 1:46185/140‑1 (MQ=255) gaAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCa > 1:78291/1‑140 (MQ=255) cggTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGtct < 2:30796/139‑1 (MQ=255) atatTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTcttctt < 2:78291/140‑1 (MQ=255) cGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGcca > 2:204193/1‑140 (MQ=255) gACTGGCTTAAATCAGCCTTAATTTCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGc > 2:264703/1‑140 (MQ=255) aCTGGCTTAAATCAGCCTTAATTTCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCATGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCa < 1:315623/140‑1 (MQ=255) cTTAAATCAGCCTTAATTTCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCt > 1:104427/1‑140 (MQ=255) tCAGCCTTAATTTCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCt < 2:104427/140‑1 (MQ=255) ttCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCtt < 1:204193/140‑1 (MQ=255) | CGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTT > NC_000913/2454480‑2454742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |