Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,493,138	G→A	P39P (CCC→CCT)	frc ←	formyl‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,493,138	0	G	A	100.0%	71.2 / NA	21	P39P (CCC→CCT)	frc	formyl‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/12);  total (9/12)

TCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCGGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGCCAGATTGCACACCGGTGAAATCGAGAACTTTAATTCCTTGAAGTGGAGTTGACATAGCAATACCTATT  >  NC_000913/2493022‑2493267                                                                                                                     |                                                                                                                                  tCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTc                                                                                                            >  1:337361/1‑140 (MQ=255)             gTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCa                                                                                                >  2:311123/1‑140 (MQ=255)                tttAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGc                                                                                             <  1:205279/140‑1 (MQ=255)                  tAACTCAATAGAACGTTTGTTACTGTTAAGCATGGGGAGGTAAAGCGCATCGATATCAGGAATATCTCGTAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCa                                                                                           >  2:267889/1‑140 (MQ=255)                          tAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCAt                                                                                       <  1:295838/136‑1 (MQ=255)                          tAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCAt                                                                                       >  2:295838/1‑136 (MQ=255)                             aaCGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTa                                                                                <  1:260124/140‑1 (MQ=255)                               cGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGTATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACa                                                                              >  1:388730/1‑140 (MQ=255)                                  ttGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCAt                                                                                       >  1:341873/1‑128 (MQ=255)                                  ttGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCAt                                                                                       <  2:341873/128‑1 (MQ=255)                                      tACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATggg                                                                       <  2:104456/140‑1 (MQ=255)                                              aGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGCCAGATTg                                                               >  1:13822/1‑140 (MQ=255)                                                 aTGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGCCAGATTGcac                                                            <  1:141662/140‑1 (MQ=255)                                                  tGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGcc                                                                     >  1:226903/1‑130 (MQ=255)                                                  tGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGcc                                                                     <  2:226903/130‑1 (MQ=255)                                                     tGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGcc                                                                     <  2:49948/127‑1 (MQ=255)                                                     tGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGcc                                                                     >  1:49948/1‑127 (MQ=255)                                                                                  tctcGCAGCTGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGCCAGATTGCACACCGGTGAAATCGAGAACTTTAATTCCTTGAAg                           <  2:337361/140‑1 (MQ=255)                                                                                           tGGTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGCCAGATTGCACACCGGTGAAATCGAGAACTTTAATTCCTTGAAGTGGAGTTGa                  <  2:388730/140‑1 (MQ=255)                                                                                            ggTGACGCGTTACGTCACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGCCAGATTGCACACCGGTGAAATCGAGAACTTTAATTCCTTGAAGTGGAGTTGAc                 <  2:258681/140‑1 (MQ=255)                                                                                                           cACCAACGCCAGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGCCAGATTGCACACCGGTGAAATCGAGAACTTTAATTCCTTGAAGTGGAGTTGACATAGCAATACCTAtt  <  1:253070/140‑1 (MQ=255)                                                                                                                     |                                                                                                                                  TCCGCTGTTTTGGTATTTAACTCAATAGAACGTTTGTTACTGTTAAGCATGGTGAAGTAAAGCGCATCGATATCAGGAATATCTCGCAGCTGGTGACGCGTTACGTCACCAACGCCGGGACGTTCAATTTTAATAACGTCAGCGCCAAACCAGGCCAGCATTTGAGTACAAGATGGGCCAGATTGCACACCGGTGAAATCGAGAACTTTAATTCCTTGAAGTGGAGTTGACATAGCAATACCTATT  >  NC_000913/2493022‑2493267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑