Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,626,342	C→T	R410C (CGC→TGC)	ppx →	exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,626,342	0	C	T	100.0%	109.2 / NA	32	R410C (CGC→TGC)	ppx	exopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/17);  total (15/17)

GATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACA  >  NC_000913/2626205‑2626476                                                                                                                                          |                                                                                                                                       gATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATAGCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTgtgc                                                                                                                                      <  2:375915/140‑1 (MQ=255)     ggCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATc                                                                                                                                  >  1:302586/1‑140 (MQ=255)         gccATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCg                                                                                                                              <  1:75052/140‑1 (MQ=255)                            ggTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTcga                                                                                                            <  1:111260/139‑1 (MQ=255)                            ggTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTcga                                                                                                            >  2:111260/1‑139 (MQ=255)                                   tatCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTcg                                                                                                             >  2:235039/1‑131 (MQ=255)                                   tatCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTcg                                                                                                             <  1:235039/131‑1 (MQ=255)                                       aaCCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACcgcg                                                                                                >  1:274130/1‑140 (MQ=255)                                        aCCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTAc                                                                                                    <  1:56811/135‑1 (MQ=255)                                        aCCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTAc                                                                                                    >  2:56811/1‑135 (MQ=255)                                        aCCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCgcgc                                                                                               <  2:279323/140‑1 (MQ=255)                                         ccACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCt                                                                                              <  2:295421/140‑1 (MQ=255)                                           acaGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCttt                                                                                            >  1:92138/1‑140 (MQ=255)                                             aGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTAc                                                                                          <  2:274130/140‑1 (MQ=255)                                                    tGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGttt                                                                                   >  2:289362/1‑140 (MQ=255)                                                              cTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATTATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCt                                                                                        <  2:270600/125‑1 (MQ=255)                                                              cTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCt                                                                                        >  1:270600/1‑125 (MQ=255)                                                              cTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAAc                                                                         >  2:193750/1‑140 (MQ=255)                                                                   cTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGtt                                                                                    <  2:288057/124‑1 (MQ=255)                                                                   cTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGtt                                                                                    >  1:288057/1‑124 (MQ=255)                                                                        atTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATGTACCGCGCTTTACGTTGTTTAAGAAGAAACAGTTGCTGcc                                                               >  2:350019/1‑140 (MQ=255)                                                                           cTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACt                                                            >  2:138800/1‑140 (MQ=255)                                                                            tGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTg                                                           >  2:129546/1‑140 (MQ=255)                                                                             gCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGa                                                          <  1:138800/140‑1 (MQ=255)                                                                              cAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGa                                                          <  2:159559/139‑1 (MQ=255)                                                                              cAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGa                                                          >  1:159559/1‑139 (MQ=255)                                                                                       gccTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTAtt                                                <  2:333222/138‑1 (MQ=255)                                                                                             ccGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCt                                          <  1:289362/140‑1 (MQ=255)                                                                                                ggTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTgg                                       >  2:287095/1‑140 (MQ=255)                                                                                                       aTCAGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTGGCGTATTa                                <  2:17787/140‑1 (MQ=255)                                                                                                          aGGAACAGCAGCTGATGATGGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTGGCGTATTACTc                             <  1:350019/140‑1 (MQ=255)                                                                                                                           atgGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTGGCGTATTACTCAACAATCAACGTCAGGc            <  2:275142/140‑1 (MQ=255)                                                                                                                           atgGCGACACTGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTGGCGTATTACTCAACAATCAACGTCAGGc            <  1:352934/140‑1 (MQ=255)                                                                                                                                     tGGTGTGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTGGCGTATTACTCAACAATCAACGTCAGGCAACCACcaca  <  2:108118/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGTAAAGCGATTAAGCTCGACGATCTACCGCGCTTTACCTTGTTTAAGAAGAAACAGTTCCTGCCACTGATACAGCTATTGCGCCTTGGCGTATTACTCAACAATCAACGTCAGGCAACCACCACA  >  NC_000913/2626205‑2626476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑