Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,681,049	T→C	N566S (AAC→AGC)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,681,049	0	T	C	100.0%	50.5 / NA	15	N566S (AAC→AGC)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

GCACTTCCTGATTGGTTGGGCAGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGTTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGACGTTCGTAAGCACTGGCGCGAATCAAACTCGCCTGTCCGCACTGCGGATTTTTGTTCAGCGCATGTGCGCGTTTCAGAGC  >  NC_000913/2680930‑2681185                                                                                                                        |                                                                                                                                         gCACTTCCTGATTGGTTGGGCAGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCGCACGc                                                                                                                      >  2:63155/1‑140 (MQ=255)      tCCTGATTGGTTGGGCAGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCg                                                                                                                           <  1:1993/130‑1 (MQ=255)      tCCTGATTGGTTGGGCAGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCg                                                                                                                           >  1:250644/1‑130 (MQ=255)      tCCTGATTGGTTGGGCAGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCg                                                                                                                           >  2:1993/1‑130 (MQ=255)      tCCTGATTGGTTGGGCAGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCg                                                                                                                           <  2:250644/130‑1 (MQ=255)               gttggGCAGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTc                                                                                                        >  1:110554/1‑140 (MQ=255)                 tggGCAGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCgg                                                                                                      <  1:63155/140‑1 (MQ=255)                      aGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCttgt                                                                                                 <  2:390091/140‑1 (MQ=255)                                     tttgGCAAAAATCCAGACCAGCGTCGAAGTTCCCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGa                                                                                  <  1:251751/139‑1 (MQ=255)                                       tgGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGACg                                                                                <  2:110554/140‑1 (MQ=255)                                                          cGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGACGTTCGTAAGCACTGGCGCGa                                                             >  1:15561/1‑140 (MQ=255)                                                                              gccgccAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGACGTTCGTAAGCACTGGCGCGAATCAAACTCGCCTGTCCGCa                                         >  2:254563/1‑140 (MQ=255)                                                                                                         ccACCGGCTTTGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGACGTTCGTAAGCACTGGCGCGAATCAAACTCGCCTGTCCGCACTGCGGATTTTTGTTCAGCGCATGTgc              >  1:163996/1‑140 (MQ=255)                                                                                                           aCCGGCTTTGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGACGTTCGTAAGCACTGGCGCGAATCAAACTCGCCTGTCCGCACTGCGGATTTTTGTTCAGCGCATGTgcgc            >  2:118621/1‑140 (MQ=255)                                                                                                                 tttGCTGCTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGACGTTCGTAAGCACTGGCGCGAATCAAACTCGCCTGTCCGCACTGCGGATTTTTGTTCAGCGCATGTGCGCGTTTCa      <  2:163996/140‑1 (MQ=255)                                                                                                                     ctgctgCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGACGTTCGTAAGCACTGGCGCGAATCAAACTCGCCTGTCCGCACTGCGGATTTTTGTTCAGCGCATGTGCGCGTTTCAGAGc  <  1:27388/140‑1 (MQ=255)                                                                                                                        |                                                                                                                                         GCACTTCCTGATTGGTTGGGCAGGCGCGAAGACTTTGTTGGCAAAAATCCAGACCAGCGTCGAAGTTACCATTACGCGCCGCCAGTCGTGCCAGGCCATAATAGCCACCGGCTTTGCTGTTGCCGCTCCAGACCGCACGCCAGAAATCCTCTTCGGCTTGTTGATATTGTCCCTGACGTTCGTAAGCACTGGCGCGAATCAAACTCGCCTGTCCGCACTGCGGATTTTTGTTCAGCGCATGTGCGCGTTTCAGAGC  >  NC_000913/2680930‑2681185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑