Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	205,795	A→G	T224A (ACC→GCC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	205,795	0	A	G	100.0%	87.5 / NA	25	T224A (ACC→GCC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/17);  total (8/17)

AGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCC  >  NC_000913/205663‑205917                                                                                                                                     |                                                                                                                           aGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTcga                                                                                                                     <  1:17528/140‑1 (MQ=255) aGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTcga                                                                                                                     <  2:17604/140‑1 (MQ=255)        tGCACGCGGCGGTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCt                                                                                                              >  2:108192/1‑140 (MQ=255)         gCACGCGGCGGTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTa                                                                                                             >  1:272933/1‑140 (MQ=255)                gcggTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACg                                                                                                         >  1:10690/1‑137 (MQ=255)                gcggTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACg                                                                                                         <  2:10690/137‑1 (MQ=255)                gcggTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGccc                                                                                                      >  2:223285/1‑140 (MQ=255)                    tGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGt                                                                                                  >  1:247198/1‑140 (MQ=255)                           ggCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCcg                                                                                                     <  2:383885/130‑1 (MQ=255)                           ggCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCcg                                                                                                     >  1:383885/1‑130 (MQ=255)                           ggCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTAtt                                                                                           <  1:366772/140‑1 (MQ=255)                               gAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTcgcc                                                                                       <  2:218072/140‑1 (MQ=255)                                aaGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTcgccg                                                                                      <  2:318950/140‑1 (MQ=255)                                 aGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTcgccgc                                                                                     <  1:108192/140‑1 (MQ=255)                                 aGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTcgccgc                                                                                     <  2:247198/140‑1 (MQ=255)                                  gcgcgcGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCgca                                                                                    <  2:95305/140‑1 (MQ=255)                                   cgcgcgGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCgcag                                                                                   <  2:272933/140‑1 (MQ=255)                                              cccGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCg                                                                        <  1:85914/140‑1 (MQ=255)                                                               cgacgTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGAtgt                                                       >  2:144728/1‑140 (MQ=255)                                                                     gcgcTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGc                                                 <  2:127436/140‑1 (MQ=255)                                                                                   gcagcGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATc                                   >  2:19204/1‑140 (MQ=255)                                                                                                    cacGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCaa                  <  1:144728/140‑1 (MQ=255)                                                                                                    cacGAAATCCGCGTCGCGATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCaa                  <  1:125711/140‑1 (MQ=255)                                                                                                                    cgATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGcc  <  1:223285/140‑1 (MQ=255)                                                                                                                    cgATCCACGACGGCTTTGCCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGcc  <  1:178748/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                           AGCTATCTGCACGCGGCGGTGGAACTGGCGGAAGCGCGCGGTTTGCCCGTCGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTGTGAGCTGTTTGCCGACATCCCCGAAGCCCTTGCCAACACCGTTGAGATCGCC  >  NC_000913/205663‑205917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑