Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,753,046	A→G	T418A (ACC→GCC)	recN →	DNA repair protein RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,753,046	0	A	G	100.0%	93.7 / NA	27	T418A (ACC→GCC)	recN	DNA repair protein RecN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/13);  total (14/13)

CTTGCACAGCTGATCACCGACAGTATGCATGCGCTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCACTGATTTTTGATGAAGT  >  NC_000913/2752917‑2753176                                                                                                                                  |                                                                                                                                   cTTGCACAGCTGATCACCGACAGTATGCATGCGCTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAgg                                                                                                                          >  1:235591/1‑140 (MQ=255)         gCTGATCACCGACAGTATGCATGCGCTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGc                                                                                                                     >  2:23051/1‑137 (MQ=255)         gCTGATCACCGACAGTATGCATGCGCTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGc                                                                                                                     <  1:23051/137‑1 (MQ=255)         gCTGATCACCGACAGTATGCATGCGCTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCaa                                                                                                                  >  2:280192/1‑140 (MQ=255)            gATCACCGACAGTATGCATGCGCTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGc                                                                                                               <  2:163868/140‑1 (MQ=255)             aTCACCGACAGTATGCATGCGCTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCa                                                                                                              >  1:177364/1‑140 (MQ=255)                               gcgcTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGc                                                                                            >  2:207393/1‑140 (MQ=255)                                cgcTCTCAATGCCTCATGGGCAGTTCACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGCAACCGCCAAACCTGGTCAGCCAATGCAGTCTATTGCCAAAGTCGCa                                                                                           >  2:293129/1‑140 (MQ=255)                                cgcTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCa                                                                                           >  2:341564/1‑140 (MQ=255)                                               aTGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATc                                                                                         >  2:353817/1‑127 (MQ=255)                                               aTGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATc                                                                                         <  1:353817/127‑1 (MQ=255)                                                      gTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCa                                                                     >  2:124159/1‑140 (MQ=255)                                                          aCGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGc                                                                 <  1:133296/140‑1 (MQ=255)                                                          aCGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGc                                                                 <  1:341564/140‑1 (MQ=255)                                                          aCGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGc                                                                 <  1:182195/140‑1 (MQ=255)                                                             atcgatGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACt                                                              <  2:102633/140‑1 (MQ=255)                                                             atcgatGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACt                                                              >  2:102825/1‑140 (MQ=255)                                                               cgatGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTgg                                                            <  1:151338/140‑1 (MQ=255)                                                                 atGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCa                                                          >  1:286817/1‑140 (MQ=255)                                                                                aGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACg                                           <  1:280192/140‑1 (MQ=255)                                                                                  cATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGgc                                         >  1:285604/1‑140 (MQ=255)                                                                                           ggCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAAt                                >  1:158368/1‑140 (MQ=255)                                                                                                  aCGGTGCCGATCGTATTGAGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAcc                         >  1:235661/1‑140 (MQ=255)                                                                                                                    aGTTTCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCACTGATTTTtgat       <  1:177091/140‑1 (MQ=255)                                                                                                                      tttCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCACTGATTTTtgatga     <  2:235591/140‑1 (MQ=255)                                                                                                                      tttCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCACTGATTTTtgatga     <  2:286817/140‑1 (MQ=255)                                                                                                                        tCGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCACTGATTTTTGATGAAg   <  1:293129/140‑1 (MQ=255)                                                                                                                         cGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCACTGATTTTTGATGAAGt  >  1:292390/1‑140 (MQ=255)                                                                                                                         cGGGTAACCGCCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCACTGATTTTTGATGAAGt  <  1:124159/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   CTTGCACAGCTGATCACCGACAGTATGCATGCGCTCTCAATGCCGCATGGGCAGTTTACGATCGATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCACTGATTTTTGATGAAGT  >  NC_000913/2752917‑2753176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑