Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,820,068	C→T	K648K (AAG→AAA)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,820,068	0	C	T	100.0%	89.1 / NA	26	K648K (AAG→AAA)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/10);  total (16/10)

GCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCA  >  NC_000913/2819943‑2820201                                                                                                                              |                                                                                                                                      gcagcaGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAg                                                                                                                         >  2:131775/1‑140 (MQ=255)               ttCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGaa                                                                                                                            <  2:64485/123‑1 (MQ=255)               ttCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGaa                                                                                                                            >  1:64485/1‑123 (MQ=255)                        gatgCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACctttcgctttcg                                                                                                  <  2:67568/140‑1 (MQ=255)                         atgCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCtttcgctttcgc                                                                                                 >  1:234682/1‑140 (MQ=255)                              gAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCtt                                                                                            >  2:110084/1‑140 (MQ=255)                                aCAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCg                                                                                          >  2:291799/1‑140 (MQ=255)                                 cAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCga                                                                                         <  1:131775/140‑1 (MQ=255)                                    aCCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGAt                                                                                      >  1:18218/1‑140 (MQ=255)                                         tatCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCatg                                                                                 >  2:189502/1‑140 (MQ=255)                                                    cGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTc                                                                      >  2:132125/1‑140 (MQ=255)                                                       cTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGAt                                                                   >  2:81565/1‑140 (MQ=255)                                                       cTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGAt                                                                   <  2:126610/140‑1 (MQ=255)                                                                   ccGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGcg                                                       <  1:110084/140‑1 (MQ=255)                                                                    cGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGcga                                                      <  1:248665/140‑1 (MQ=255)                                                                    cGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGcga                                                      >  2:17809/1‑140 (MQ=255)                                                                          acaACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATc                                                <  1:189502/140‑1 (MQ=255)                                                                              cTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAAt                                                           >  2:202031/1‑125 (MQ=255)                                                                              cTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAAt                                                           <  1:202031/125‑1 (MQ=255)                                                                                         aaTCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCAcc                                    <  1:258327/137‑1 (MQ=255)                                                                                         aaTCGCCCATGCTCAGCACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCAcc                                    >  2:258327/1‑137 (MQ=255)                                                                                                    cTCAGCACGCGTACGCGCCCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGa                          <  1:76632/136‑1 (MQ=255)                                                                                                    cTCAGCACGCGTACGCGCCCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGa                          >  2:76632/1‑136 (MQ=255)                                                                                                         cACGCGTACGCGCTCATCATATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGaa                 >  1:134523/1‑140 (MQ=255)                                                                                                                      tcatcaTATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGttt    >  2:255614/1‑140 (MQ=255)                                                                                                                        atcatATTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCa  >  1:229257/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                      GCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCA  >  NC_000913/2819943‑2820201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑