Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,855,402	T→C	R355R (CGT→CGC)	fhlA →	DNA‑binding transcriptional activator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,855,402	0	T	C	100.0%	68.5 / NA	20	R355R (CGT→CGC)	fhlA	DNA‑binding transcriptional activator FhlA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

GCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAA  >  NC_000913/2855270‑2855516                                                                                                                                     |                                                                                                                   gCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAg                                                                                                             >  2:357326/1‑140 (MQ=255)     ggCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACg                                                                                                         >  1:141566/1‑140 (MQ=255)                                    aaGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGag                                                                          <  2:23435/140‑1 (MQ=255)                                    aaGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGag                                                                          <  2:182989/140‑1 (MQ=255)                                         tttACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGc                                                                                    >  2:25349/1‑125 (MQ=255)                                         tttACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGc                                                                                    <  1:25349/125‑1 (MQ=255)                                                  aCCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGt                                                            <  1:357326/140‑1 (MQ=255)                                                   ccAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAAGCTCGCCCTGACCGAGCAGCTCAACAATGtt                                                           >  2:242960/1‑140 (MQ=255)                                                   ccAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGtt                                                           >  1:63057/1‑140 (MQ=255)                                                      aTCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGAt                                                        >  1:196976/1‑140 (MQ=255)                                                        cTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAg                                                      >  2:101213/1‑140 (MQ=255)                                                                aCTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTg                                              >  1:206763/1‑140 (MQ=255)                                                                 cTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTgg                                             <  1:101213/140‑1 (MQ=255)                                                                                   cGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAg                           <  2:141566/140‑1 (MQ=255)                                                                                     tgtgGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCg                         <  1:242960/140‑1 (MQ=255)                                                                                       tgGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGaa                       <  1:191989/140‑1 (MQ=255)                                                                                                 tGTCGATAACGCCCTCCCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCCGACCGAGCAGCTCAACAATGTTGATAGTGAAATTTGGGGGATTATTTGCCGCAGCGAAGCCATGTACa             >  2:199630/1‑140 (MQ=255)                                                                                                            cccTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTaaa  <  2:196976/140‑1 (MQ=255)                                                                                                             ccTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAg            >  1:335755/1‑129 (MQ=255)                                                                                                             ccTCGCCTATCAGGAAATCCATCGCCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAg            <  2:335755/129‑1 (MQ=255)                                                                                                                                     |                                                                                                                   GCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATGTTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTGCTTAAA  >  NC_000913/2855270‑2855516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑