Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,015,001	A→G	R19R (CGT→CGC)	yqeC ←	uncharacterized protein YqeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,015,001	0	A	G	100.0%	136.9 / NA	38	R19R (CGT→CGC)	yqeC	uncharacterized protein YqeC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/21);  total (17/21)

CAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATCCATCAGGAAGTAACGCAATTATCAGGCGTTATTAG  >  NC_000913/3014867‑3015136                                                                                                                                       |                                                                                                                                        cAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGttt                                                                                                                                    >  2:36661/1‑140 (MQ=255)         cGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCAc                                                                                                                            >  1:120957/1‑140 (MQ=255)                   aaCATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAAt                                                                                                                  <  2:120957/140‑1 (MQ=255)                             tcgtcgtcgTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGcc                                                                                                        >  2:176207/1‑140 (MQ=255)                             tcgtcgtcgTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGcc                                                                                                        >  2:261421/1‑140 (MQ=255)                              cgtcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCg                                                                                                       >  1:247490/1‑140 (MQ=255)                                 cgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATg                                                                                                    <  1:36661/140‑1 (MQ=255)                                  gtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATgg                                                                                                   <  2:247490/140‑1 (MQ=255)                                  gtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATgg                                                                                                   <  1:261421/140‑1 (MQ=255)                                  gtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATgg                                                                                                   >  1:338174/1‑140 (MQ=255)                                   tcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATggg                                                                                                  <  2:282793/140‑1 (MQ=255)                                         ttAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACt                                                                                            <  1:141769/140‑1 (MQ=255)                                            acacacGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACtata                                                                                         <  2:164751/140‑1 (MQ=255)                                               cacGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGt                                                                                                 <  1:386470/129‑1 (MQ=255)                                               cacGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGt                                                                                                 >  2:386470/1‑129 (MQ=255)                                                  gCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTc                                                                                   >  2:19481/1‑140 (MQ=255)                                                  gCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTc                                                                                   >  1:195605/1‑140 (MQ=255)                                                       ccGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAg                                                                              >  1:19921/1‑140 (MQ=255)                                                       ccGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAg                                                                              >  1:99293/1‑140 (MQ=255)                                                       ccGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAg                                                                              <  2:647/140‑1 (MQ=255)                                                                       gTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAg                                                                              <  1:136277/124‑1 (MQ=255)                                                                       gTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAg                                                                              >  2:43064/1‑124 (MQ=255)                                                                       gTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAg                                                                              >  2:136277/1‑124 (MQ=255)                                                                       gTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAg                                                                              <  1:43064/124‑1 (MQ=255)                                                                        ttCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc                                                                    >  1:35567/1‑133 (MQ=255)                                                                        ttCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc                                                                    <  2:35567/133‑1 (MQ=255)                                                                             ccagccaAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGa                                                                     >  2:290862/1‑127 (MQ=255)                                                                             ccagccaAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGa                                                                     <  1:290862/127‑1 (MQ=255)                                                                                 ccaAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAtt                                                    <  2:19921/140‑1 (MQ=255)                                                                                 ccaAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAtt                                                    <  2:99293/140‑1 (MQ=255)                                                                                      aaaGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATccc                                               <  2:338174/140‑1 (MQ=255)                                                                                               tGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAAt                                      <  2:195605/140‑1 (MQ=255)                                                                                                            aGCACCAACAACAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATCCATCAGGAAGTa                         <  1:183763/140‑1 (MQ=255)                                                                                                                 caacaaCAGAAATCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATCCATCAGGAAGTAACGCa                    >  2:103027/1‑140 (MQ=255)                                                                                                                          aaaTCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATCCATCAGGAAGTAACGCAATTATCAgg           <  2:121653/140‑1 (MQ=255)                                                                                                                          aaaTCACAGTGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATCCATCAGGAAGTAACGCAATTATCAgg           <  1:187674/140‑1 (MQ=255)                                                                                                                                   tGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATCCATCAGGAAGTAACGCAATTATCAGGCGTTATTAg  <  1:44525/140‑1 (MQ=255)                                                                                                                                   tGGGGCGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATCCATCAGGAAGTAACGCAATTATCAGGCGTTATTAg  >  1:344796/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATCCATCAGGAAGTAACGCAATTATCAGGCGTTATTAG  >  NC_000913/3014867‑3015136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑