Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,057,148	A→G	intergenic (+157/+30)	sibC → / ← serA	small RNA SibC/phosphoglycerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,057,148	0	A	G	100.0%	77.9 / NA	22	intergenic (+157/+30)	sibC/serA	small RNA SibC/phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

TAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCA  >  NC_000913/3057020‑3057275                                                                                                                                 |                                                                                                                                tAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGTAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTg                                                                                                                      <  2:378521/140‑1 (MQ=255)     tCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGAttt                                                                                                                  >  1:100708/1‑140 (MQ=255)         cATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCag                                                                                                              >  2:200792/1‑140 (MQ=255)          aTTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCaga                                                                                                             >  2:366828/1‑140 (MQ=255)              aataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAg                                                                                                         >  1:204899/1‑140 (MQ=255)               ataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgg                                                                                                        <  2:86542/140‑1 (MQ=255)                    cacaACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAAt                                                                                                   <  2:100708/140‑1 (MQ=255)                         cGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgg                                                                                                        >  1:317835/1‑130 (MQ=255)                         cGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgg                                                                                                        <  2:317835/130‑1 (MQ=255)                              tGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagca                                                                                         >  2:308352/1‑140 (MQ=255)                                        ttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGcgcg                                                                               <  2:124426/140‑1 (MQ=255)                                          gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGaa                                                                             <  1:200792/140‑1 (MQ=255)                                                                                      ggCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCGGCGCTTTTTCGGCa                                 <  2:35559/140‑1 (MQ=255)                                                                                                    gaAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGc                   <  2:335452/140‑1 (MQ=255)                                                                                                     aaGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTTGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCt                  >  1:272351/1‑140 (MQ=255)                                                                                                        ggATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtca               >  2:142996/1‑140 (MQ=255)                                                                                                         gATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaa              <  2:204899/140‑1 (MQ=255)                                                                                                                 aaCGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatat           >  1:368622/1‑135 (MQ=255)                                                                                                                 aaCGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatat           <  2:368622/135‑1 (MQ=255)                                                                                                                  aCGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATaa     <  1:142996/140‑1 (MQ=255)                                                                                                                     ggCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTTGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACca  <  2:272351/140‑1 (MQ=255)                                                                                                                     ggCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACca  <  1:366828/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                TAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCA  >  NC_000913/3057020‑3057275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑