Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,109,589	T→C	L13L (TTA→CTA)	yqgA →	DUF554 domain‑containing protein YqgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,109,589	0	T	C	100.0%	76.9 / NA	22	L13L (TTA→CTA)	yqgA	DUF554 domain‑containing protein YqgA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/9);  total (13/9)

CTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAA  >  NC_000913/3109454‑3109716                                                                                                                                        |                                                                                                                                cTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACt                                                                                                                             <  2:343996/140‑1 (MQ=255)                 aGGACTTT‑‑GAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGgcgc                                                                                                           <  1:300580/140‑1 (MQ=255)                        tGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAGTAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGc                                                                                                      >  1:273017/1‑140 (MQ=255)                        tGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGc                                                                                                      >  1:233676/1‑140 (MQ=255)                        tGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGc                                                                                                      >  2:100295/1‑140 (MQ=255)                                   aaGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCACACTGCTCAGCCAACGc                                                                                           <  2:392817/140‑1 (MQ=255)                                       cATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTAc                                                                                       <  2:183296/140‑1 (MQ=255)                                                    aaaCGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCcg                                                                          <  2:233676/140‑1 (MQ=255)                                                                   gAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATc                                                           >  2:252826/1‑140 (MQ=255)                                                                    aaaaTCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCa                                                          >  2:125184/1‑140 (MQ=255)                                                                                   aGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCa                                           <  1:164009/140‑1 (MQ=255)                                                                                   aGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCa                                           <  1:125184/140‑1 (MQ=255)                                                                                   aGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGATAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCa                                           <  2:97031/140‑1 (MQ=255)                                                                                          aGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTgg                                    >  2:250512/1‑140 (MQ=255)                                                                                          aGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTgg                                    >  1:213558/1‑140 (MQ=255)                                                                                                    gtgGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTAt                          >  2:972/1‑140 (MQ=255)                                                                                                         cATCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTAc                     <  1:972/140‑1 (MQ=255)                                                                                                          aTCGGCCCTTTTATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACt                    >  2:310726/1‑140 (MQ=255)                                                                                                                  ttttATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGa            >  1:7139/1‑140 (MQ=255)                                                                                                                   tttATCAACGCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGaa           >  1:282024/1‑140 (MQ=255)                                                                                                                            gCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCaa  >  1:294902/1‑140 (MQ=255)                                                                                                                            gCTAGTGCAGTCCTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCaa  >  1:224011/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                CTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAA  >  NC_000913/3109454‑3109716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑