Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,150,856	T→C	I130V (ATA→GTA)	exbD ←	Ton complex subunit ExbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,150,856	0	T	C	100.0%	83.0 / NA	24	I130V (ATA→GTA)	exbD	Ton complex subunit ExbD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/16);  total (8/16)

CTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCTTCGGTTAACGCATTCAACGCCGTAATCAT  >  NC_000913/3150740‑3150988                                                                                                                     |                                                                                                                                     cTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCa                                                                                                               <  2:105926/140‑1 (MQ=255)    cAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCg                                                                                                            <  1:334328/140‑1 (MQ=255)              aaGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAc                                                                                                  <  2:257185/140‑1 (MQ=255)                    cAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcat                                                                                            <  1:285483/140‑1 (MQ=255)                       cATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCaa                                                                                         <  2:367352/140‑1 (MQ=255)                           ttGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtc                                                                                     >  1:201305/1‑140 (MQ=255)                                        tGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACgg                                                                        >  2:210424/1‑140 (MQ=255)                                                  atgaGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTc                                                                 <  2:297835/137‑1 (MQ=255)                                                  atgaGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTc                                                                 >  1:297835/1‑137 (MQ=255)                                                        gTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaa                                                        <  2:123928/140‑1 (MQ=255)                                                            cgcATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGaagaag                                                    >  1:252149/1‑140 (MQ=255)                                                                tCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCCACGTCTCGTAATCGACGGGTTTGTCCGCTCGGAAGAAGAtgg                                                >  2:164517/1‑140 (MQ=255)                                                                 cAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt                                               <  1:115648/140‑1 (MQ=255)                                                                 cAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt                                               <  2:201305/140‑1 (MQ=255)                                                                   ggCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggtgg                                             <  1:163902/140‑1 (MQ=255)                                                                    gCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga                                                      >  1:148310/1‑130 (MQ=255)                                                                    gCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga                                                      <  2:187079/130‑1 (MQ=255)                                                                    gCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga                                                      <  2:148310/130‑1 (MQ=255)                                                                    gCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga                                                      >  1:187079/1‑130 (MQ=255)                                                                                 acttCGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTTCCt                               <  1:210424/140‑1 (MQ=255)                                                                                     cGCTTTGGCGGTTTCTTCGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCTTCgg                           <  2:5292/140‑1 (MQ=255)                                                                                                      cGCCGACCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCTTCGGTTAACGCATTCAACGcc          >  1:372362/1‑140 (MQ=255)                                                                                                           aCCAGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCTTCGGTTAACGCATTCAACGCCGTAAt     <  2:383562/140‑1 (MQ=255)                                                                                                              aGACCTACCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCTTCGGTTAACGCATTCAACGCCGTAATCAt  <  2:220198/140‑1 (MQ=255)                                                                                                                     |                                                                                                                                     CTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGTGTCTTTCTTGCCTTCGGTTAACGCATTCAACGCCGTAATCAT  >  NC_000913/3150740‑3150988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑