Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,900,306	(A)9→8	intergenic (‑33/‑286)	ygcW ← / → yqcE	putative deoxygluconate dehydrogenase/putative transport protein YqcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,900,298	0	A	.	100.0%	133.7 / NA	32	intergenic (‑25/‑294)	ygcW/yqcE	putative deoxygluconate dehydrogenase/putative transport protein YqcE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (16/16);  total (16/16)

TGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTA  >  NC_000913/2900168‑2900427                                                                                                                                   |                                                                                                                                  tGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCaaaaaaaaga                                                                                                                          <  2:35993/140‑3 (MQ=255) tGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCaaaaaaaaga                                                                                                                          <  1:597399/140‑3 (MQ=255)          ccTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACt                                                                                                                <  1:735880/140‑1 (MQ=255)             ggCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTcc                                                                                                             <  1:281777/140‑1 (MQ=255)             ggCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTcc                                                                                                             >  1:776766/1‑140 (MQ=255)             ggCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTcc                                                                                                             >  1:597089/1‑140 (MQ=255)              gCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTccc                                                                                                            >  2:566202/1‑140 (MQ=255)               ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTcccc                                                                                                           <  2:180492/140‑1 (MQ=255)                   aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCcgcg                                                                                                       >  1:87785/1‑140 (MQ=255)                       gCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGagag                                                                                                   <  2:785400/140‑1 (MQ=255)                        cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                  >  1:233960/1‑140 (MQ=255)                                aCCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCgaga                                                                                                    >  2:454053/1‑130 (MQ=255)                                aCCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCgaga                                                                                                    <  1:454053/130‑1 (MQ=255)                                   ggTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCtt                                                                                       >  1:122633/1‑140 (MQ=255)                                              cGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtct                                                                            <  2:233960/140‑1 (MQ=255)                                                     aCCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGACTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTACCTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTaa                                                                     <  2:556888/140‑1 (MQ=255)                                                           cAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGgag                                                               >  1:629267/1‑140 (MQ=255)                                                           cAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGgag                                                               >  1:298980/1‑140 (MQ=255)                                                                         ccATTGAGAACGCATTGAGAGATTCTATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCAt                                                 <  2:774915/140‑1 (MQ=255)                                                                         ccATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCAt                                                 <  1:566202/140‑1 (MQ=255)                                                                         ccATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAACTCTGGTAACTGGAGATAATTTTTAGCAt                                                 <  1:432368/140‑1 (MQ=255)                                                                            ttGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAg                                              >  1:522369/1‑140 (MQ=255)                                                                              gagaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg                                            <  2:87785/140‑1 (MQ=255)                                                                                 aaCGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAt                                         <  2:122633/140‑1 (MQ=255)                                                                                      aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTg                                    >  2:711587/1‑140 (MQ=255)                                                                                              aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                            >  2:452681/1‑140 (MQ=255)                                                                                              aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                            <  1:312577/140‑1 (MQ=255)                                                                                              aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgtg                            <  1:188762/140‑1 (MQ=255)                                                                                                tCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGAt                          >  1:682031/1‑140 (MQ=255)                                                                                                  gATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCg                        <  2:597089/140‑1 (MQ=255)                                                                                                                  tCCAATTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGata        >  2:518206/1‑140 (MQ=255)                                                                                                                     aaTTTCCGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTGTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTc     >  2:560177/1‑140 (MQ=255)                                                                                                                           cGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTa  <  2:597259/137‑1 (MQ=255)                                                                                                                           cGTAAGGC‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTa  >  1:597259/1‑137 (MQ=255)                                                                                                                                   |                                                                                                                                  TGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTA  >  NC_000913/2900168‑2900427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑