Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,018,976	(A)8→7	intergenic (+39/+37)	matP → / ← ompA	macrodomain Ter protein/outer membrane porin A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,018,969	0	A	.	100.0%	85.3 / NA	21	intergenic (+32/+44)	matP/ompA	macrodomain Ter protein/outer membrane porin A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/11);  total (10/11)

TGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATC  >  NC_000913/1018853‑1019094                                                                                                                     |                                                                                                                              tGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGt                                                                                                       >  2:225009/1‑140 (MQ=255)    aGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGtttt                                                                                                    <  2:159662/140‑1 (MQ=255)         cGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTAc                                                                                               <  1:224991/140‑1 (MQ=255)                cAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACga                                                                                        <  1:183260/140‑1 (MQ=255)                              cgAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGc                                                                          <  1:504362/140‑1 (MQ=255)                                aaTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGATAACTTAAGCCTGCgg                                                                        <  1:540757/140‑1 (MQ=255)                                aaTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCgg                                                                        <  2:750068/140‑1 (MQ=255)                                      aTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGt                                                                  >  2:546374/1‑140 (MQ=255)                                         tcttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTAc                                                               >  1:799413/1‑140 (MQ=255)                                                            tGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTa                                            >  2:565225/1‑140 (MQ=255)                                                                           gTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAcg                             <  1:225009/140‑1 (MQ=255)                                                                                 aaTAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAcg                             <  2:451971/134‑1 (MQ=255)                                                                                 aaTAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAcg                             >  1:451971/1‑134 (MQ=255)                                                                                 aaTAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGcgacga                       >  1:655015/1‑140 (MQ=255)                                                                                     aCTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCg                   >  2:253832/1‑140 (MQ=255)                                                                                          cGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGcc              >  2:407782/1‑140 (MQ=255)                                                                                                   gTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGATGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCg     >  2:501270/1‑140 (MQ=255)                                                                                                    ttCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGa    >  2:334639/1‑140 (MQ=255)                                                                                                     tCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGAt   <  1:101450/140‑1 (MQ=255)                                                                                                      cAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATc  <  2:230230/140‑1 (MQ=255)                                                                                                      cAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATc  <  2:571843/140‑1 (MQ=255)                                                                                                                     |                                                                                                                              TGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATC  >  NC_000913/1018853‑1019094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑