Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,423,893	(A)6→5	coding (112/1458 nt)	trkG →	Rac prophage; K(+) transporter TrkG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,423,888	0	A	.	100.0%	142.6 / NA	34	coding (107/1458 nt)	trkG	Rac prophage; K(+) transporter TrkG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (21/13);  total (21/13)

TTATAATTGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGTTTT  >  NC_000913/1423758‑1424019                                                                                                                                   |                                                                                                                                    ttATAATTGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCtg                                                                                                                           >  2:441721/1‑140 (MQ=255)       ttGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCtgtttgtt                                                                                                                     >  1:660338/1‑140 (MQ=255)               atatTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTtat                                                                                                             >  2:465411/1‑140 (MQ=255)                                 aTCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATAttttt                                                                                           >  1:695668/1‑140 (MQ=255)                                   ctcATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATAttttttt                                                                                         >  2:488123/1‑140 (MQ=255)                                      aTGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCa                                                                                      >  1:598620/1‑140 (MQ=255)                                             agTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATtggtgg                                                                               >  2:347678/1‑140 (MQ=255)                                             agTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATtggtgg                                                                               <  2:129897/140‑1 (MQ=255)                                               ttgttACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCg                                                                             <  1:292140/140‑1 (MQ=255)                                                 gttACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGa                                                                           >  2:309747/1‑140 (MQ=255)                                                   tACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATT‑GTGGCGGAGCg                                                                        <  2:660338/140‑1 (MQ=255)                                                    aCTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCg                                                                        <  1:43790/140‑1 (MQ=255)                                                    aCTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCg                                                                        <  1:809469/140‑1 (MQ=255)                                                        atatGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGt                                                                    >  1:682094/1‑140 (MQ=255)                                                           tgtgtgGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGtata                                                                 <  1:501963/140‑1 (MQ=255)                                                            gtgtgGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATAc                                                                >  1:675186/1‑140 (MQ=255)                                                            gtgtgGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATAc                                                                >  2:217394/1‑140 (MQ=255)                                                             tgtgGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCATGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACa                                                               >  2:688986/1‑140 (MQ=255)                                                               tgGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCg                                                                             <  1:767433/124‑1 (MQ=255)                                                               tgGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCg                                                                             >  2:767433/1‑124 (MQ=255)                                                                  gTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTaa                                                          <  1:137313/140‑1 (MQ=255)                                                                          tttGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTg                                                  <  2:695668/140‑1 (MQ=255)                                                                             ggCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCa                                               <  2:675186/140‑1 (MQ=255)                                                                                         tttCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTa                                   >  2:774007/1‑140 (MQ=255)                                                                                         tttCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTa                                   >  1:241141/1‑140 (MQ=255)                                                                                                    ccaccaATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATggg                        <  1:774007/140‑1 (MQ=255)                                                                                                    ccaccaATGGTTGTAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATggg                        <  1:362017/140‑1 (MQ=255)                                                                                                                 tAGCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTg           >  1:192334/1‑140 (MQ=255)                                                                                                                   gCATTGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTa         <  2:595331/140‑1 (MQ=255)                                                                                                                      ttGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATg      >  2:601368/1‑140 (MQ=255)                                                                                                                      ttGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATg      >  2:269039/1‑140 (MQ=255)                                                                                                                       tGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGt     >  2:436836/1‑140 (MQ=255)                                                                                                                       tGTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTCTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGt     >  2:7578/1‑140 (MQ=255)                                                                                                                        gTTTTATAAAG‑AAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGG‑‑TACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGtttt  >  1:269372/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                    TTATAATTGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGTTTT  >  NC_000913/1423758‑1424019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑