Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,326	(T)9→8	intergenic (+55/‑51)	dtpA → / → gstA	dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,318	0	T	.	100.0%	68.1 / NA	17	intergenic (+47/‑59)	dtpA/gstA	dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/8);  total (9/8)

ATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGA  >  NC_000913/1714191‑1714437                                                                                                                                |                                                                                                                        aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAACCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCa                                                                                                            >  2:488593/1‑140 (MQ=255)      gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAc                                                                                                       >  1:518302/1‑140 (MQ=255)        tgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGc                                                                                                     <  1:520454/140‑1 (MQ=255)          cccGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAc                                                                                                   <  2:153591/139‑1 (MQ=255)            cgcgCCGAAACTGCCCCGGATGACGCAGGATGCCGCGGCAGAAAAAGCGGCGAAAGCGGCCGTAGCGTAAAATTCAGGAAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTa                                                                                                 <  2:168456/140‑1 (MQ=255)            cgcgCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTa                                                                                                 >  2:773233/1‑140 (MQ=255)                                             cGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAcc                                                                >  2:59354/1‑140 (MQ=255)                                             cGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTAcc                                                                >  2:630418/1‑140 (MQ=255)                                                           cggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCt                                                  <  1:657199/140‑1 (MQ=255)                                                            ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTa                                                 >  1:131122/1‑140 (MQ=255)                                                                                   aTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCg                          <  1:59354/140‑1 (MQ=255)                                                                                     tCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCt                        <  2:131122/140‑1 (MQ=255)                                                                                              ctctTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATc               >  2:248975/1‑140 (MQ=255)                                                                                               tctTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCCCTCTCGCTTCCCATATCa              <  1:432373/140‑1 (MQ=255)                                                                                                 ttttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCAcc            <  2:787006/140‑1 (MQ=255)                                                                                                           cGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTgaga  >  2:201683/1‑140 (MQ=255)                                                                                                           cGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTgaga  >  1:169448/1‑140 (MQ=255)                                                                                                                                |                                                                                                                        ATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGA  >  NC_000913/1714191‑1714437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑