Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	136,457	(T)8→7	coding (8/867 nt)	speE ←	spermidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	136,450	0	T	.	100.0%	313.9 / NA	73	coding (15/867 nt)	speE	spermidine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (38/35);  total (38/35)

CGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAG  >  NC_000913/136311‑136582                                                                                                                                            |                                                                                                                                     cGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGt                                                                                                                                      >  2:546612/1‑140 (MQ=255)   ccATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGttt                                                                                                                                    >  1:631123/1‑140 (MQ=255)       ccAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGttttttt                                                                                                                                <  2:55344/140‑1 (MQ=255)           cgcCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtttttttcggc                                                                                                                            <  2:313970/140‑5 (MQ=255)             ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCCTGCCACTG‑TTTTTTTCGGCCa                                                                                                                         <  2:574525/140‑1 (MQ=255)             ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtttttttcggc                                                                                                                            >  2:357578/1‑134 (MQ=255)             ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGtttttttcggc                                                                                                                            <  1:357578/138‑5 (MQ=255)             ccATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCa                                                                                                                         <  1:34940/140‑1 (MQ=255)                ttACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATgg                                                                                                                      >  1:340095/1‑140 (MQ=255)                ttACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATgg                                                                                                                      >  2:663370/1‑140 (MQ=255)                        ccAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAc                                                                                                              <  2:631123/140‑1 (MQ=255)                        ccAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAc                                                                                                              >  2:233134/1‑140 (MQ=255)                          aaaTGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcct                                                                                                            <  1:700162/140‑1 (MQ=255)                          aaaTGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcct                                                                                                            <  1:233134/140‑1 (MQ=255)                           aaTGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctc                                                                                                           >  1:342237/1‑140 (MQ=255)                           aaTGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctc                                                                                                           >  2:740068/1‑140 (MQ=255)                             tGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                         <  1:576430/140‑1 (MQ=255)                             tGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                         <  2:340095/140‑1 (MQ=255)                                  cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGtt                                                                                                    >  1:542070/1‑140 (MQ=255)                                  cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGtt                                                                                                    >  1:789117/1‑140 (MQ=255)                                  cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGtt                                                                                                    <  1:663370/140‑1 (MQ=255)                                      ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                         >  1:382993/1‑131 (MQ=255)                                      ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATAcctcct                                                                                                         <  2:382993/131‑1 (MQ=255)                                      ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAaca                                                                                                >  2:366622/1‑140 (MQ=255)                                       tcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAacac                                                                                               >  2:499247/1‑140 (MQ=255)                                                aTCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTaaaaa                                                                                      >  1:358678/1‑140 (MQ=255)                                                 tCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTaa                                                                                                  <  2:217466/127‑1 (MQ=255)                                                 tCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTaa                                                                                                  >  1:217466/1‑127 (MQ=255)                                                       ccTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                               <  1:149341/140‑1 (MQ=255)                                                         tGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCa                                                                             <  2:633527/140‑1 (MQ=255)                                                           gTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAAc                                                                           >  2:478831/1‑140 (MQ=255)                                                              aTCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                                        <  2:288458/140‑1 (MQ=255)                                                               tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAggg                                                                                 <  1:121464/130‑1 (MQ=255)                                                               tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAggg                                                                                 >  2:121464/1‑130 (MQ=255)                                                               tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcgc                                                                              >  2:532145/1‑133 (MQ=255)                                                               tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcgc                                                                              <  1:532145/133‑1 (MQ=255)                                                               tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACataa                                                                       >  1:689609/1‑140 (MQ=255)                                                               tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAAAGGGCGCAACataat                                                                      <  1:376604/140‑1 (MQ=255)                                                               tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGT‑AAAAAAGGGCGCAACataat                                                                      <  1:359979/140‑1 (MQ=255)                                                                cGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACataat                                                                      >  2:789531/1‑140 (MQ=255)                                                                  gTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAg                                                                    >  1:609683/1‑140 (MQ=255)                                                                     ttttCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCAGTAAAAAAAGGGCGCAACATAATAGCTa                                                                 >  1:777057/1‑140 (MQ=255)                                                                     ttttCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTAGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTa                                                                 >  1:294078/1‑140 (MQ=255)                                                                             aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGAc                                                         <  1:98226/140‑1 (MQ=255)                                                                                 aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTAGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgcg                                                     >  1:695729/1‑140 (MQ=255)                                                                                   aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGa                                                   >  1:242589/1‑140 (MQ=255)                                                                                   aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGa                                                   >  2:756257/1‑140 (MQ=255)                                                                                   aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGa                                                   >  1:550070/1‑140 (MQ=255)                                                                                   aaCGTTATCTACCGCAAAGTACTACCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGa                                                   >  2:269139/1‑140 (MQ=255)                                                                                     cGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATg                                                 >  1:525350/1‑140 (MQ=255)                                                                                      gTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGc                                                <  1:756257/140‑1 (MQ=255)                                                                                       ttATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                               <  2:570494/140‑1 (MQ=255)                                                                                       ttATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                               <  2:689609/140‑1 (MQ=255)                                                                                       ttATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                               <  2:342237/140‑1 (MQ=255)                                                                                        tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCAc                                              >  1:798106/1‑140 (MQ=255)                                                                                        tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCAc                                              >  2:678416/1‑140 (MQ=255)                                                                                             aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCa                                         <  2:609683/140‑1 (MQ=255)                                                                                               cGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                               >  2:639385/1‑132 (MQ=255)                                                                                               cGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                               <  1:639385/132‑1 (MQ=255)                                                                                                gCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAata                                      >  2:564955/1‑140 (MQ=255)                                                                                                 cAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAatat                                     <  2:542070/140‑1 (MQ=255)                                                                                                 cAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAatat                                     <  1:678416/140‑1 (MQ=255)                                                                                                      tACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTa                                >  1:323941/1‑140 (MQ=255)                                                                                                         tGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaa                             >  2:808273/1‑140 (MQ=255)                                                                                                          gCCCAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaa                            <  1:499247/140‑1 (MQ=255)                                                                                                           cccAAACTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACaaaa                           <  2:798106/140‑1 (MQ=255)                                                                                                                aCTGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTa                      >  1:435108/1‑140 (MQ=255)                                                                                                                  tGGTCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATc                    <  1:808273/140‑1 (MQ=255)                                                                                                                     tCGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAgg                 <  2:323941/140‑1 (MQ=255)                                                                                                                      cGTGTAGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAggg                >  2:653132/1‑140 (MQ=255)                                                                                                                           aGCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattat           <  2:495286/140‑1 (MQ=255)                                                                                                                            gCGTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGattatt          <  1:478831/140‑1 (MQ=255)                                                                                                                              gTTTCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTg        >  1:364258/1‑140 (MQ=255)                                                                                                                               tttCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAGGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGa       >  2:583328/1‑140 (MQ=255)                                                                                                                               tttCATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGa       <  2:457114/140‑1 (MQ=255)                                                                                                                                  cATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAgg                 >  1:279152/1‑127 (MQ=255)                                                                                                                                  cATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAgg                 <  2:279152/127‑1 (MQ=255)                                                                                                                                  cATGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGt    >  2:138804/1‑140 (MQ=255)                                                                                                                                    tGCCACTG‑TTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAg  <  1:564955/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     CGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGATTATTTGACGTAG  >  NC_000913/136311‑136582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑