Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	383,661	(T)7→8	coding (275/1197 nt)	yaiP ←	putative glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	383,654	1	.	T	96.7%	207.3 / ‑0.7	60	coding (282/1197 nt)	yaiP	putative glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base T (27/31);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTA‑TTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGAT  >  NC_000913/383515‑383784                                                                                                                                             |                                                                                                                                   cGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTa                                                                                                                                     >  2:598523/1‑140 (MQ=255)    cACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTA‑ttt                                                                                                                                 >  1:774232/1‑140 (MQ=255)       cgcgCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTA‑tttttt                                                                                                                              >  1:314038/1‑140 (MQ=255)        gcgcTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTA‑ttttttt                                                                                                                             >  1:251415/1‑140 (MQ=255)         cgcTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTA‑tttttttt                                                                                                                            >  2:220275/1‑139 (MQ=255)             cAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTA‑ttttttttgcgt                                                                                                                        <  1:346248/140‑6 (MQ=255)               aTTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGa                                                                                                                       <  2:774232/140‑1 (MQ=255)                        aGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGcaacaa                                                                                                              >  2:26629/1‑140 (MQ=255)                          cATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAg                                                                                                            >  1:417738/1‑140 (MQ=255)                              taGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGt                                                                                                        <  1:220275/140‑1 (MQ=255)                                gCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCg                                                                                                      <  1:552067/140‑1 (MQ=255)                                    aTTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGcccc                                                                                                  >  1:617938/1‑140 (MQ=255)                                    aTTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGcccc                                                                                                  >  1:310853/1‑140 (MQ=255)                                      tCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCAt                                                                                                >  1:232236/1‑140 (MQ=255)                                      tCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCAt                                                                                                >  1:56319/1‑140 (MQ=255)                                          tCTTGATCGGGCGGAACATAGGGGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGc                                                                                            >  1:204904/1‑140 (MQ=255)                                           cTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCg                                                                                           >  2:627889/1‑140 (MQ=255)                                             tGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGtt                                                                                         <  2:366944/140‑1 (MQ=255)                                               aTCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTg                                                                                       >  2:28955/1‑140 (MQ=255)                                                  gggCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACt                                                                                    >  1:168796/1‑140 (MQ=255)                                                       gAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGc                                                                               <  1:26629/140‑1 (MQ=255)                                                             aGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCTCCATTTGCGTTTGACTTCCGCCATGAc                                                                         <  2:136199/140‑1 (MQ=255)                                                             aGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGAc                                                                         <  2:208754/140‑1 (MQ=255)                                                             aGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGAc                                                                         >  2:283934/1‑140 (MQ=255)                                                               gtgtCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCg                                                                       <  1:627889/140‑1 (MQ=255)                                                                 gtCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCt                                                                     <  1:547605/140‑1 (MQ=255)                                                                 gtCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCt                                                                     <  1:453958/140‑1 (MQ=255)                                                                 gtCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCt                                                                     <  2:755389/140‑1 (MQ=255)                                                                  tCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCtt                                                                    >  2:106429/1‑140 (MQ=255)                                                                  tCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCtt                                                                    >  2:646116/1‑140 (MQ=255)                                                                  tCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCtt                                                                    >  1:269460/1‑140 (MQ=255)                                                                  tCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTA‑TTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTc                                                                   >  1:225401/1‑140 (MQ=255)                                                                    ggCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACATTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCg                                                                  >  2:640386/1‑140 (MQ=255)                                                                      cATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTA‑TTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTa                                                               <  2:225401/140‑1 (MQ=255)                                                                         cACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTAtt                                                             <  1:28955/140‑1 (MQ=255)                                                                         cACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACATTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTAtt                                                             <  1:640386/140‑1 (MQ=255)                                                                              aaaaaaaCCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGAc                                                                         >  2:429977/1‑123 (MQ=255)                                                                              aaaaaaaCCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGAc                                                                         <  1:429977/123‑1 (MQ=255)                                                                                     ccTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGcc                                                 >  1:113884/1‑140 (MQ=255)                                                                                     ccTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGcc                                                 >  1:685246/1‑140 (MQ=255)                                                                                      cTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCcg                                                >  2:304744/1‑140 (MQ=255)                                                                                                   gCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCaaa                                   >  2:662969/1‑140 (MQ=255)                                                                                                       aaTTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTAc                               <  2:310853/140‑1 (MQ=255)                                                                                                            aGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGc                          <  1:662969/140‑1 (MQ=255)                                                                                                             ggCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCa                         <  1:283934/140‑1 (MQ=255)                                                                                                              gCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTTACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCaa                        >  2:323962/1‑140 (MQ=255)                                                                                                                  ttCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAAt                    <  2:510486/140‑1 (MQ=255)                                                                                                                  ttCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAAt                    <  2:149397/140‑1 (MQ=255)                                                                                                                  ttCATCAGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAAt                    >  2:665793/1‑140 (MQ=255)                                                                                                                       caGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTTACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAggg               <  1:323962/140‑1 (MQ=255)                                                                                                                       caGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAggg               <  1:352311/140‑1 (MQ=255)                                                                                                                       caGCGCACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAggg               <  2:232236/140‑1 (MQ=255)                                                                                                                         gcgcACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGtt             <  1:106429/140‑1 (MQ=255)                                                                                                                         gcgcACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGtt             <  1:159656/140‑1 (MQ=255)                                                                                                                         gcgcACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGtt             <  2:196041/140‑1 (MQ=255)                                                                                                                          cgcACCACCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTa            <  1:94640/140‑1 (MQ=255)                                                                                                                            caccacCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACg          >  2:762061/1‑140 (MQ=255)                                                                                                                            caccacCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACg          >  1:592779/1‑140 (MQ=255)                                                                                                                            caccacCTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACg          <  1:598523/140‑1 (MQ=255)                                                                                                                             accaccTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGc         <  1:646116/140‑1 (MQ=255)                                                                                                                             accaccTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGc         >  1:786300/1‑140 (MQ=255)                                                                                                                               caccTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTCGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGc         <  1:332221/138‑1 (MQ=255)                                                                                                                               caccTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTCGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGc         >  2:332221/1‑138 (MQ=255)                                                                                                                                accTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGt      <  2:685246/140‑1 (MQ=255)                                                                                                                                 ccTTTACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTa     >  2:94138/1‑140 (MQ=255)                                                                                                                                   tttACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATTACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGa   <  2:617938/140‑1 (MQ=255)                                                                                                                                    ttACCGGTATTTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGAt  <  1:382781/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                   CGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTA‑TTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGAT  >  NC_000913/383515‑383784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑