Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,594,256 (C)8→9 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,594,2481.C97.4% 133.6 / ‑3.3 39pseudogene (3839/5422 nt)yneOAIDA‑I family autotransporter YneO
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (24/14);  total (24/15)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CACGCAGTG‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTGGAG  >  NC_000913/1594116‑1594382
                                                                                                                                      |                                                                                                                                      
cacGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑cccccc                                                                                                                                  >  1:653249/1‑140 (MQ=255)
          ccccccccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGatat                                                                                                                         <  1:141043/140‑1 (MQ=255)
                 cAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCcgtcgt                                                                                                                  >  2:675887/1‑140 (MQ=255)
                  aGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTa                                                                                                                 <  1:741157/140‑1 (MQ=255)
                   ggCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTAc                                                                                                                >  2:482172/1‑140 (MQ=255)
                        aaCGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCcgtcg                                                                                                                   >  1:794038/1‑132 (MQ=255)
                        aaCGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCcgtcg                                                                                                                   <  2:794038/132‑1 (MQ=255)
                               atatCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATAcgccg                                                                                                    <  1:403492/140‑1 (MQ=255)
                                  tCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCgg                                                                                                 <  1:482172/140‑1 (MQ=255)
                                     ccTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGaa                                                                                               <  1:159847/139‑1 (MQ=255)
                                     ccTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGaa                                                                                               >  2:159847/1‑139 (MQ=255)
                                     ccTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAAt                                                                                              >  2:79703/1‑140 (MQ=255)
                                              gtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTaa                                                                                     >  1:244295/1‑140 (MQ=255)
                                                          gACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATcc                                                                         <  2:275166/140‑1 (MQ=255)
                                                                 cacCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTAcc                                                                  >  1:108709/1‑140 (MQ=255)
                                                                            tGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACTCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTca                                                       >  2:308597/1‑140 (MQ=255)
                                                                                   tAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCacc                                                >  1:427286/1‑140 (MQ=255)
                                                                                        tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctac                                           <  2:297911/140‑1 (MQ=255)
                                                                                        tgtTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctac                                           >  2:719076/1‑140 (MQ=255)
                                                                                          ttGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctaccg                                         >  2:11252/1‑140 (MQ=255)
                                                                                          ttGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgctaccg                                         >  1:522920/1‑140 (MQ=255)
                                                                                             aCTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTg                                      >  1:548813/1‑140 (MQ=255)
                                                                                             aCTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGCACCCAGTTTTGTCACATCACCGCTACCGCTg                                      >  1:59854/1‑140 (MQ=255)
                                                                                                  ccATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACt                                 >  1:482541/1‑140 (MQ=255)
                                                                                                       ttAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCg                            >  2:730577/1‑140 (MQ=255)
                                                                                                           tGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCAt                        >  2:157153/1‑140 (MQ=255)
                                                                                                            ggCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATc                       >  1:746433/1‑140 (MQ=255)
                                                                                                            ggCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATc                       >  2:33054/1‑140 (MQ=255)
                                                                                                                 agTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa                  >  2:561165/1‑140 (MQ=255)
                                                                                                                 agTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa                  >  1:329718/1‑140 (MQ=255)
                                                                                                                 agTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCa                  >  2:558578/1‑140 (MQ=255)
                                                                                                                  gTCGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAg                 <  1:79703/140‑1 (MQ=255)
                                                                                                                    cGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCt               <  2:329718/140‑1 (MQ=255)
                                                                                                                    cGGAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCt               <  1:11252/140‑1 (MQ=255)
                                                                                                                     ggAACCGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTa              >  2:693955/1‑140 (MQ=255)
                                                                                                                         ccGAAAGCAATTT‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTc         <  1:623374/140‑1 (MQ=255)
                                                                                                                          cGAAAGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTc         <  1:719076/140‑1 (MQ=255)
                                                                                                                            aaaGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAg       <  2:746433/140‑1 (MQ=255)
                                                                                                                            aaaGCAATTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAg       >  1:554597/1‑140 (MQ=255)
                                                                                                                                 aaTTTCCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTGgag  <  2:244295/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CACGCAGTG‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTGGAG  >  NC_000913/1594116‑1594382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑