| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,143,684 | (G)6→7 | coding (419/3318 nt) | dgcE → | putative diguanylate cyclase DgcE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,143,678 | 1 | . | G | 93.5% | 98.0 / 0.3 | 31 | coding (413/3318 nt) | dgcE | putative diguanylate cyclase DgcE |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (19/10); total (20/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ACGACCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATC > NC_000913/2143545‑2143809 | acgacCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCAGTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑gggggg < 2:358189/140‑1 (MQ=255) acCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑gggggggtg > 2:748944/1‑137 (MQ=255) acCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑gggggggtg > 2:626920/1‑137 (MQ=255) cATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑gggggggtgtt < 2:126615/140‑6 (MQ=255) aaTATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTgg > 2:62204/1‑140 (MQ=255) aaTATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTgg > 2:446522/1‑140 (MQ=255) gttgAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTcc > 1:314032/1‑140 (MQ=255) gttgAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTGCTGGTTGTcc > 1:554905/1‑140 (MQ=255) aGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCctgctg > 2:445890/1‑140 (MQ=255) gCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGa > 2:408249/1‑140 (MQ=255) gCCGTGCTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTTCACAATCTGCCTGTCTGGCTGCCTCTCGCACTCGGCAGCGCCGTTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGa > 2:30626/1‑140 (MQ=255) tCGGGGCGGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGga < 2:801041/140‑1 (MQ=255) tACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAg < 1:438864/140‑1 (MQ=255) aCGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAgg < 1:748944/140‑1 (MQ=255) cGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAggg < 1:62204/140‑1 (MQ=255) tAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCa > 1:254863/1‑140 (MQ=255) ctgcCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa > 2:430490/1‑140 (MQ=255) tgcCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGat > 1:523249/1‑140 (MQ=255) gcCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGTCCGGAGACGATCCTCTCAGGGCATTTTTGata < 2:554905/140‑1 (MQ=255) ttGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAAt > 1:58646/1‑140 (MQ=255) tGCAAAATCTGGCTGACTGGCTGCGTCTGGTACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc > 1:485203/1‑140 (MQ=255) tGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc > 2:154311/1‑140 (MQ=255) tGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc > 1:782225/1‑140 (MQ=255) tGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc > 1:345888/1‑140 (MQ=255) tGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc > 2:353041/1‑140 (MQ=255) aTCTGGCTGACTGGCTGCGTCTGGTACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCgg < 2:485203/140‑1 (MQ=255) aTCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCgg < 1:445890/140‑1 (MQ=255) tGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCAc > 2:40152/1‑140 (MQ=255) tCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCg > 2:714940/1‑140 (MQ=255) cgggCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGc < 2:340333/138‑1 (MQ=255) cTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGc < 2:314032/140‑1 (MQ=255) tCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGAtt < 1:446522/140‑1 (MQ=255) cATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaaa < 1:408249/140‑1 (MQ=255) cATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaaa > 1:127826/1‑140 (MQ=255) ccTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATc > 2:420871/1‑140 (MQ=255) ccTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATc > 1:185570/1‑140 (MQ=255) | ACGACCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATC > NC_000913/2143545‑2143809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |