| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,220,451 | +C | coding (1539/2298 nt) | bglX ← | beta‑D‑glucoside glucohydrolase, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,220,447 | 1 | . | C | 97.9% | 162.1 / ‑5.1 | 48 | M515V (ATG→GTG) | bglX | beta‑D‑glucoside glucohydrolase, periplasmic |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (22/24); minor base G (1/0); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCAGCGCCAGCGGACGCCCGTTCATCAGCACCAGCACCAGCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑T‑CCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATACTGATTCAGG > NC_000913/2220317‑2220571 | aCCAGCGCCAGCGGACGCCCGTTCATCAGCACCAGCACCAGCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑T‑ccccctgtg > 2:131105/1‑135 (MQ=255)aCCAGCGCCAGCGGACGCCCGTTCATCAGCACCAGCACCAGCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑T‑ccccctgtg < 2:299772/140‑6 (MQ=255) gcgccagcgGACGCCCGTTCATCAGCACCAGCACCAGCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGACTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTc < 1:772272/140‑1 (MQ=255) cgccagcgGACGCCCGTTCATCAGCACCAGCACCAGCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCa > 1:142510/1‑140 (MQ=255) cgccagcgGACGCCCGTTCATCAGCACCAGCACCAGCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCa > 2:89770/1‑140 (MQ=255) gCCCGTTCATCAGCACCAGCACCAGCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCACC‑CCCCTGTGCTTCACCGACTACAGc < 1:422928/140‑1 (MQ=255) gCCCGTTCATCAGCACCAGCACCAGCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGc < 1:89770/140‑1 (MQ=255) caccagcaccagCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCa > 2:502815/1‑140 (MQ=255) gcaccagcGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTg > 1:724081/1‑140 (MQ=255) agcGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCg > 2:5923/1‑140 (MQ=255) agcGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCg > 2:175011/1‑140 (MQ=255) gcGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGc > 1:204247/1‑140 (MQ=255) tttACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCGA‑TGCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTc > 1:692156/1‑140 (MQ=255) ttACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCt > 1:304876/1‑140 (MQ=255) tGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGc > 2:549299/1‑140 (MQ=255) ttCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTtcat < 2:793351/140‑1 (MQ=255) ttCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTtcat > 2:373396/1‑140 (MQ=255) cgccgcAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCatcaatc < 1:373396/140‑1 (MQ=255) ccgcAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAatcat > 2:18499/1‑140 (MQ=255) ccgcAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAatcat > 1:517840/1‑140 (MQ=255) cAATCAAGTCACGCTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCAtctc > 2:89387/1‑140 (MQ=255) aGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTgcgg < 1:39957/140‑1 (MQ=255) aCGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGaa < 2:517840/140‑1 (MQ=255) gTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAAcg < 1:175011/140‑1 (MQ=255) cTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGAt > 1:718044/1‑140 (MQ=255) tAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGAc < 1:254509/140‑1 (MQ=255) gTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCg < 1:194238/140‑1 (MQ=255) gTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCg < 1:502815/140‑1 (MQ=255) gTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCg < 2:707355/140‑1 (MQ=255) gTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCg < 1:233684/140‑1 (MQ=255) gTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCg < 2:718044/140‑1 (MQ=255) gTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCg < 2:364480/140‑1 (MQ=255) gTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGATTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCg < 2:724081/140‑1 (MQ=255) gTGATACCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCg < 1:731042/140‑1 (MQ=255) tGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGc < 1:549299/140‑1 (MQ=255) gATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGct < 2:692156/140‑1 (MQ=255) gATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGct < 2:304876/140‑1 (MQ=255) gTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGAc > 2:419618/1‑129 (MQ=255) gTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGAc < 1:419618/129‑1 (MQ=255) gTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCAt > 2:435922/1‑140 (MQ=255) gTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCAt > 1:681796/1‑140 (MQ=255) gTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCAt < 2:142510/140‑1 (MQ=255) gTCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCAt > 1:754475/1‑140 (MQ=255) tCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATa < 1:18499/140‑1 (MQ=255) tCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATa < 1:531990/140‑1 (MQ=255) tCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATa > 1:587233/1‑140 (MQ=255) tCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATa < 2:204247/140‑1 (MQ=255) tCCGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATa > 1:793246/1‑140 (MQ=255) ccGGCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATAc > 2:41089/1‑140 (MQ=255) gCTGGAGGCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATACTGa > 1:729894/1‑140 (MQ=255) ggCTTCGTGCGCCA‑TCCCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATACTGATTCAgg < 2:754475/140‑1 (MQ=255) | ACCAGCGCCAGCGGACGCCCGTTCATCAGCACCAGCACCAGCGGTTTACCGGTGGCTTTCAGCGCCGCAATCAAGTCACGTTGGCTTTGCGGAATAGTGATATCGGTCCGGCTGGAGGCTTCGTGCGCCA‑T‑CCCCTGTGCTTCACCGACTACAGCCACCACCACATCAGATTGTTTCGCCGTCTGCACCGCTTCATCAATCATCTCTTGCGGCGAACGCGGATCGACTTTGACCGCTTCTTCATACTGATTCAGG > NC_000913/2220317‑2220571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |