Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,288,561	(C)7→8	pseudogene (354/2525 nt)	yejO ←	adhesin‑like autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,288,554	1	.	C	100.0%	127.8 / NA	39	pseudogene (361/2525 nt)	yejO	adhesin‑like autotransporter YejO
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/22);  total (17/22)

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATT  >  NC_000913/2288415‑2288679                                                                                                                                             |                                                                                                                              aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACgg                                                                                                                                <  2:458254/140‑1 (MQ=255)     agaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑cccc                                                                                                                           >  2:627956/1‑140 (MQ=255)      gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑ccccc                                                                                                                          >  1:398424/1‑140 (MQ=255)                tCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCtttt                                                                                                                 >  2:179245/1‑140 (MQ=255)                  cGAATCCCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTc                                                                                                               <  1:378553/140‑1 (MQ=255)                         cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGAtt                                                                                                        >  1:298975/1‑140 (MQ=255)                               ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAgcgc                                                                                                  <  1:758538/140‑1 (MQ=255)                               ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAgcgc                                                                                                  <  1:277666/140‑1 (MQ=255)                                gCCCGCGTGGTTGTTTTAATAGCGCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTATTCCTCCCTTTGCGATAGCGACAAAGACGG‑CCCCCACATCTTTTTCAACGACTAAGcgccc                                                                                                <  2:668907/140‑1 (MQ=255)                                    gcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGcgcccgcc                                                                                             >  1:185480/1‑140 (MQ=255)                                          ttgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAAt                                                                                       <  1:731612/140‑1 (MQ=255)                                            gttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                     <  1:226445/140‑1 (MQ=255)                                              tttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                   <  1:143818/140‑1 (MQ=255)                                              tttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                   <  1:171423/140‑1 (MQ=255)                                                    aGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCa                                                                             >  1:216762/1‑140 (MQ=255)                                                           ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                      <  2:44870/140‑1 (MQ=255)                                                             tGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgcg                                                                    <  1:8532/140‑1 (MQ=255)                                                                            cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc                                                            >  1:135080/1‑133 (MQ=255)                                                                            cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc                                                            <  2:135080/133‑1 (MQ=255)                                                                             gcaaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                    <  2:216762/140‑1 (MQ=255)                                                                              caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                    <  2:806522/139‑1 (MQ=255)                                                                              caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                    >  1:806522/1‑139 (MQ=255)                                                                                         ccccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                        <  2:398424/140‑1 (MQ=255)                                                                                          cccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATCAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAg                                       <  1:708425/140‑1 (MQ=255)                                                                                            cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg                                     >  2:344718/1‑140 (MQ=255)                                                                                                ctTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGt                                 <  2:336180/140‑1 (MQ=255)                                                                                                   tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTg                              <  2:110086/140‑1 (MQ=255)                                                                                                   tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTg                              >  1:367556/1‑140 (MQ=255)                                                                                                          cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                       >  2:780849/1‑140 (MQ=255)                                                                                                           agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                        >  2:182877/1‑122 (MQ=255)                                                                                                           agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                        <  1:182877/122‑1 (MQ=255)                                                                                                           agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                      >  1:409871/1‑140 (MQ=255)                                                                                                           aggcatcctccCTTTTCTATAACAACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                      <  2:367556/135‑1 (MQ=255)                                                                                                             tagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgcg                    <  2:409871/140‑1 (MQ=255)                                                                                                                tcctccCTTTTCGATAGCGACAATTACGGCCCCCCCCATCTTTTTCAACGACTAAGCTCCCGCTTTTAAACGTGGTATCATTCGCTGCGCTATAAACCTGTAGCACACCATGATTGAGCTGAGTCTTGTTTGCCTCGccc                 >  2:480086/1‑139 (MQ=255)                                                                                                                  ctccCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcc               >  2:520591/1‑140 (MQ=255)                                                                                                                  ctccCTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcc               >  2:773527/1‑140 (MQ=255)                                                                                                                    cccTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCa             <  1:475415/140‑1 (MQ=255)                                                                                                                    cccTTTTCGATAGCGACAAAGACGGCCCCCCCCATCTTTTTAAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCa             >  2:629687/1‑140 (MQ=255)                                                                                                                           cGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc      <  1:344718/140‑1 (MQ=255)                                                                                                                           cGATAGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc      <  1:336839/140‑1 (MQ=255)                                                                                                                               aGCGACAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAAtt  >  2:218925/1‑140 (MQ=255)                                                                                                                                   aCAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt            <  2:318374/126‑1 (MQ=255)                                                                                                                                   aCAAAGACGGCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt            >  1:318374/1‑126 (MQ=255)                                                                                                                                             |                                                                                                                              ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATT  >  NC_000913/2288415‑2288679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑