Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,588	A→G	K117K (AAA→AAG)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,588	0	A	G	100.0%	170.0 / NA	48	K117K (AAA→AAG)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

CAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTC  >  NC_000913/8462‑8720                                                                                                                               |                                                                                                                                     cAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATg                                                                                                                         <  2:571484/140‑1 (MQ=255)   aaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGTGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACaa                                                                                                                              >  2:325341/1‑133 (MQ=255)   aaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGTGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACaa                                                                                                                              <  1:325341/133‑1 (MQ=255)   aaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCt                                                                                                                       <  2:615522/140‑1 (MQ=255)   aaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCt                                                                                                                       <  1:455458/140‑1 (MQ=255)     cTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCt                                                                                                                       >  2:324506/1‑138 (MQ=255)     cTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCt                                                                                                                       <  1:324506/138‑1 (MQ=255)          aGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTAGTATTa                                                                                                                >  1:749467/1‑140 (MQ=255)              aaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCaa                                                                                                            <  1:154112/140‑1 (MQ=255)              aaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCaa                                                                                                            <  2:52724/140‑1 (MQ=255)              aaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCaa                                                                                                            <  1:354766/140‑1 (MQ=255)                 atTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGa                                                                                                         <  1:598787/140‑1 (MQ=255)                  ttGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGAt                                                                                                        >  2:468734/1‑140 (MQ=255)                    ggTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCg                                                                                                      >  2:239440/1‑140 (MQ=255)                      tCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTa                                                                                                    >  2:553006/1‑140 (MQ=255)                      tCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGACGCTGGTATTAGCAACGATCGTa                                                                                                    >  1:444156/1‑140 (MQ=255)                        tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTAtt                                                                                                  >  2:117425/1‑140 (MQ=255)                                      cTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGCATTAGCAACGATCGTATTCTGATCAAACTGGc                                                                                    <  2:687672/140‑1 (MQ=255)                                         gTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTc                                                                                 >  2:531006/1‑140 (MQ=255)                                               ggCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTg                                                                           >  2:53647/1‑140 (MQ=255)                                                gCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTgg                                                                          <  1:201698/140‑1 (MQ=255)                                                 ccGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGc                                                                         <  1:117425/140‑1 (MQ=255)                                                   gTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAg                                                                       >  1:53794/1‑140 (MQ=255)                                                      tctcAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGt                                                                    <  1:239440/140‑1 (MQ=255)                                                        tcAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTAt                                                                  <  1:39320/140‑1 (MQ=255)                                                         cAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTAGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATc                                                                 <  2:749467/140‑1 (MQ=255)                                                           aCTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCg                                                               <  1:737976/140‑1 (MQ=255)                                                              gAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtgc                                                            >  2:774832/1‑140 (MQ=255)                                                              gAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtgc                                                            >  1:250364/1‑140 (MQ=255)                                                                  ttGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCa                                                        >  2:183611/1‑140 (MQ=255)                                                                     aTGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGaa                                                     <  1:211679/140‑1 (MQ=255)                                                                        cgcgTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAg                                                  <  2:53794/140‑1 (MQ=255)                                                                             cTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGa                                             <  1:536934/140‑1 (MQ=255)                                                                                       acacCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGGAGAACAGCTGGAAAAAGAAGGc                                   >  1:572397/1‑140 (MQ=255)                                                                                       acacCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGc                                   >  2:531452/1‑140 (MQ=255)                                                                                       acacCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGc                                   >  2:566597/1‑140 (MQ=255)                                                                                         acCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCAt                                 >  1:731766/1‑140 (MQ=255)                                                                                         acCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCAt                                 <  1:531006/140‑1 (MQ=255)                                                                                         acCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCAt                                 <  1:757386/140‑1 (MQ=255)                                                                                         acCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCAt                                 <  1:774832/140‑1 (MQ=255)                                                                                           cgcaGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCa                               >  2:334115/4‑140 (MQ=255)                                                                                           cGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCa                               >  1:329800/1‑140 (MQ=255)                                                                                             aaGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAAGGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAAc                             >  2:721629/1‑140 (MQ=255)                                                                                             aaGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCAt                                 <  1:692723/136‑1 (MQ=255)                                                                                             aaGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCAt                                 >  2:692723/1‑136 (MQ=255)                                                                                             aaGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAAc                             >  1:502485/1‑140 (MQ=255)                                                                                              aGCGTCAATTGCGAAAGCAAAACGCCTGATCAAGCTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACt                            <  2:641448/140‑1 (MQ=255)                                                                                                                        tGATCAAGCTCTACAACGACGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTc  >  1:596569/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                     CAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTC  >  NC_000913/8462‑8720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑