Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	140,964	A→G	W88R (TGG→CGG)	gcd ←	quinoprotein glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	140,964	0	A	G	100.0%	247.3 / NA	69	W88R (TGG→CGG)	gcd	quinoprotein glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/35);  total (34/35)

GCTAATCAGCAGTGCGACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCAGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAGGGT  >  NC_000913/140833‑141098                                                                                                                                    |                                                                                                                                       gCTAATCAGCAGTGCGACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGa                                                                                                                                <  1:54057/140‑1 (MQ=255)  cTAATCAGCAGTGCGACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGaa                                                                                                                               >  2:509216/1‑140 (MQ=255)  cTAATCAGCAGTGCGACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGaa                                                                                                                               >  2:561478/1‑140 (MQ=255)               cGACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAg                                                                                                                  >  2:1099/1‑140 (MQ=255)               cGACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAg                                                                                                                  >  2:251410/1‑140 (MQ=255)               cGACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAg                                                                                                                  >  1:335633/1‑140 (MQ=255)               cGACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAg                                                                                                                  >  1:313669/1‑140 (MQ=255)                gACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGa                                                                                                                 <  2:131279/140‑1 (MQ=255)                  ccaccaGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACg                                                                                                               <  1:731224/140‑1 (MQ=255)                    accaGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGACCGGAAGTCGAAACCAACTTCCCAGACGcc                                                                                                             >  2:496962/1‑140 (MQ=255)                       aGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCa                                                                                                          <  2:632777/140‑1 (MQ=255)                            ggCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCa                                                                                                     >  1:778142/1‑140 (MQ=255)                              cAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATg                                                                                                   >  2:409687/1‑140 (MQ=255)                              cAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATg                                                                                                   >  2:560781/1‑140 (MQ=255)                                         cTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGagcag                                                                                        <  1:143321/140‑1 (MQ=255)                                           ggCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAgcagca                                                                                      <  1:560781/140‑1 (MQ=255)                                              aGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAggg                                                                                   >  1:35226/1‑140 (MQ=255)                                                          gCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGcc                                                                       >  2:758519/1‑140 (MQ=255)                                                              cGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAg                                                                   >  1:37744/1‑140 (MQ=255)                                                              cGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAg                                                                   >  1:55818/1‑140 (MQ=255)                                                                 cAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAgcgc                                                                <  2:262357/140‑1 (MQ=255)                                                                 cAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAgcgc                                                                <  2:335633/140‑1 (MQ=255)                                                                  aGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAgcgcg                                                               <  1:251410/140‑1 (MQ=255)                                                                    aCAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAgcgcggc                                                             <  1:509216/140‑1 (MQ=255)                                                                             aGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACt                                                    >  1:177023/1‑140 (MQ=255)                                                                              ggATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAgcgcgg                                                              <  1:407940/129‑1 (MQ=255)                                                                              ggATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAgcgcgg                                                              >  2:407940/1‑129 (MQ=255)                                                                                 tCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTgcgc                                                <  1:493524/140‑1 (MQ=255)                                                                                    gCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCcac                                             >  1:214295/1‑140 (MQ=255)                                                                                     ccAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCcaca                                            <  2:55818/140‑1 (MQ=255)                                                                                     ccAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCcaca                                            <  2:37744/140‑1 (MQ=255)                                                                                     ccAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCcaca                                            <  2:214295/140‑1 (MQ=255)                                                                                         aTGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCAt                                        >  1:780573/1‑140 (MQ=255)                                                                                          tGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATc                                       >  2:786474/1‑140 (MQ=255)                                                                                          tGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATc                                       >  2:476835/1‑140 (MQ=255)                                                                                               aagaagACCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGc                                  >  2:499441/1‑140 (MQ=255)                                                                                                 gaagaCCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGa                                <  2:779599/140‑1 (MQ=255)                                                                                                   agaCCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCGGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACg                              <  2:221130/140‑1 (MQ=255)                                                                                                   agaCCAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACg                              <  1:1099/140‑1 (MQ=255)                                                                                                       cAGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGa                          <  1:192333/140‑1 (MQ=255)                                                                                                        aGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAg                         <  1:553943/140‑1 (MQ=255)                                                                                                        aGAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAg                         <  1:5956/140‑1 (MQ=255)                                                                                                         gAATGTCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAGCCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGc                        >  2:125738/1‑140 (MQ=255)                                                                                                              tCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATcac                   >  2:620387/1‑140 (MQ=255)                                                                                                              tCGCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATcac                   >  1:325277/1‑140 (MQ=255)                                                                                                                gCTGCGCGGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACaa                 >  2:10908/1‑140 (MQ=255)                                                                                                                    cgcgGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGcc             >  1:519855/1‑140 (MQ=255)                                                                                                                    cgcgGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGcc             <  2:177023/140‑1 (MQ=255)                                                                                                                    cgcgGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGcc             >  2:440613/1‑140 (MQ=255)                                                                                                                    cgcgGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGcc             <  2:398317/140‑1 (MQ=255)                                                                                                                    cgcgGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGcc             >  1:167083/1‑140 (MQ=255)                                                                                                                      cgGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAg           <  2:325277/140‑1 (MQ=255)                                                                                                                      cgGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAg           <  1:10908/140‑1 (MQ=255)                                                                                                                      cgGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAg           <  2:174791/140‑1 (MQ=255)                                                                                                                      cgGAGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAg           <  2:167083/140‑1 (MQ=255)                                                                                                                         aGTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGa        <  2:530175/140‑1 (MQ=255)                                                                                                                          gTCAGCGCCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGAt       <  1:119135/140‑1 (MQ=255)                                                                                                                              gcgcCCGGAAGTCGAAGCCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAggg   <  1:125738/140‑1 (MQ=255)                                                                                                                              gcgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAggg   >  2:58587/1‑140 (MQ=255)                                                                                                                              gcgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAggg   >  2:86734/1‑140 (MQ=255)                                                                                                                              gcgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAggg   >  2:279805/1‑140 (MQ=255)                                                                                                                              gcgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAggg   <  1:58587/140‑1 (MQ=255)                                                                                                                              gcgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAggg   <  1:561478/140‑1 (MQ=255)                                                                                                                              gcgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAggg   >  1:460412/1‑140 (MQ=255)                                                                                                                              gcgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAggg   <  1:440613/140‑1 (MQ=255)                                                                                                                              gcgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAggg   <  1:279805/140‑1 (MQ=255)                                                                                                                               cgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAGGgt  >  1:86424/1‑140 (MQ=255)                                                                                                                               cgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAGGgt  <  1:77801/140‑1 (MQ=255)                                                                                                                               cgcCCGGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAGGgt  >  1:321660/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       GCTAATCAGCAGTGCGACCACCAGTGCGGCAACTGCGCCGCTGGCAGGAATGACCAGGCGACGCCAGACAAACGGCAGGATCAGCCAGATGCCGAAGAAGACCAGAATGTCGCTGCGCGGAGTCAGCGCCCAGAAGTCGAAACCAACTTCCCAGACGCCCCAAATCATGGTGCCGAGCAGCAGGGCTGCGTATAGCCAAAGCGCGGCGCGTTTACTGCGCCACAGCATCCAGGCGACGCCGAGCATCACAAGGCCAGCGATAGGGT  >  NC_000913/140833‑141098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑