Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,605,253	G→A	C148Y (TGC→TAC)	hyfD →	hydrogenase 4 component D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,605,253	0	G	A	100.0%	104.0 / NA	32	C148Y (TGC→TAC)	hyfD	hydrogenase 4 component D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/21);  total (11/21)

CACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTG  >  NC_000913/2605129‑2605374                                                                                                                             |                                                                                                                          cacCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGAt                                                                                                            <  2:259224/140‑1 (MQ=255)         tAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGttatt                                                                                                    <  1:569622/140‑1 (MQ=255)              gCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCag                                                                                               >  1:162986/1‑140 (MQ=255)               cACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCaga                                                                                              >  1:808762/1‑140 (MQ=255)                 cGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGc                                                                                            >  2:190618/1‑140 (MQ=255)                                cATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATa                                                                                        >  1:304069/1‑129 (MQ=255)                                cATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATa                                                                                        <  2:304069/129‑1 (MQ=255)                                   ttttACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAgcg                                                                                   >  1:26383/1‑131 (MQ=255)                                   ttttACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAgcg                                                                                   <  2:26383/131‑1 (MQ=255)                                          ggTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTaa                                                                   <  2:700401/140‑1 (MQ=255)                                            tgtTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTaaaa                                                                 <  1:515415/140‑1 (MQ=255)                                               ttATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCg                                                              <  2:230355/140‑1 (MQ=255)                                                          aTGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCAc                                                   <  1:156407/140‑1 (MQ=255)                                                          aTGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCAc                                                   <  1:634662/140‑1 (MQ=255)                                                                   cTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCgg                                          <  2:162986/140‑1 (MQ=255)                                                                   cTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCgg                                          <  2:487512/140‑1 (MQ=255)                                                                   cTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCgg                                          <  1:165353/140‑1 (MQ=255)                                                                     ggTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCt                                        <  2:781837/140‑1 (MQ=255)                                                                          tctcCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTg                                   <  1:709079/140‑1 (MQ=255)                                                                          tctcCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTg                                   <  2:577182/140‑1 (MQ=255)                                                                             cctcGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTgggg                                <  1:619654/140‑1 (MQ=255)                                                                              ctcGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGt                                     >  1:113312/1‑134 (MQ=255)                                                                              ctcGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGt                                     <  2:113312/134‑1 (MQ=255)                                                                              ctcGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTggggg                               >  1:781217/1‑139 (MQ=255)                                                                              ctcGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGt                               <  1:190618/140‑1 (MQ=255)                                                                                cGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTg                                   <  1:610016/134‑1 (MQ=255)                                                                                cGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGTGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTg                                   >  2:610016/1‑134 (MQ=255)                                                                                        ctCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTTTCtttc                     >  2:272216/1‑138 (MQ=255)                                                                                        ctCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTgc                     >  2:662573/1‑140 (MQ=255)                                                                                              cAGTTGTTGTTTTTTGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTgccgccgc               <  2:646755/140‑1 (MQ=255)                                                                                                           ttGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTg  <  2:697859/140‑1 (MQ=255)                                                                                                           ttGAAATTACAGGCGGCTACTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGGTGTATCTTTCCGCCGCCACGCTGtttttt  >  2:86658/1‑139 (MQ=255)                                                                                                                             |                                                                                                                          CACCCGCATAACGGCACGAATCGTTATTACGCATTTTTACTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGACGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACAGGCGGCTGCTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAAGCGCAGCGTTCAGCACTAAAAGCGTTACTTATCACTCATATCGGCTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTTTTTG  >  NC_000913/2605129‑2605374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑