Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,644,744	G→A	H41Y (CAC→TAC)	ndk ←	nucleoside diphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,644,744	0	G	A	100.0%	70.4 / NA	21	H41Y (CAC→TAC)	ndk	nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/8);  total (13/8)

TGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCATTTTTACCTCT  >  NC_000913/2644614‑2644874                                                                                                                                   |                                                                                                                                   tGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCAtttt                                                                                                                           <  1:312857/140‑1 (MQ=255)        cGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCca                                                                                                                    >  1:7207/1‑140 (MQ=255)         gTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCcaa                                                                                                                   <  1:267813/140‑1 (MQ=255)             tCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAAt                                                                                                               >  2:686529/1‑140 (MQ=255)                  ttCCAGCACGGAAACCACGCTCGGTCAAGAGGACATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGa                                                                                                          >  2:195965/1‑140 (MQ=255)                                        gggCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAgcgcgc                                                                                    <  1:323419/140‑1 (MQ=255)                                                    aTGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTAcc                                                                        >  1:43148/1‑140 (MQ=255)                                                    aTGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTAcc                                                                        >  2:793359/1‑140 (MQ=255)                                                                aGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGttttt                                                            >  2:44295/1‑140 (MQ=255)                                                                  aCCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTg                                                          >  2:43255/1‑140 (MQ=255)                                                                           gAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGt                                                 <  1:686529/140‑1 (MQ=255)                                                                            aaCGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGtt                                                <  2:769955/140‑1 (MQ=255)                                                                                gTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGt                                            >  1:802496/1‑140 (MQ=255)                                                                                  tttCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGttt                                          >  1:438038/1‑140 (MQ=255)                                                                                        tCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTgatgat                                    <  1:43255/140‑1 (MQ=255)                                                                                             ttCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGaaa                               >  2:328898/1‑140 (MQ=255)                                                                                                       aaGCCACGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTc                     >  1:422558/1‑140 (MQ=255)                                                                                                            aCGTGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAg                >  1:20192/1‑140 (MQ=255)                                                                                                               tGCCTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCa             >  2:37899/1‑140 (MQ=255)                                                                                                                 ccTGTTCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCAtt           >  2:749405/1‑140 (MQ=255)                                                                                                                     ttCAACGGTCAGGTACAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCATTTTTa       <  2:43148/140‑1 (MQ=255)                                                                                                                          cGGTCAGGTACAGCATTTTGGTGCCAACAATTCTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCATTTTTACctct  <  2:7207/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                   TGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGACGTTTTTTGCTACCGCGTTCGGTTTGATGATGGAAAAAGTACGTTCAATAGCCATTTTTACCTCT  >  NC_000913/2644614‑2644874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑