Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,732,474	T→C	H567R (CAC→CGC)	clpB ←	ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,474	0	T	C	100.0%	102.1 / NA	30	H567R (CAC→CGC)	clpB	ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

TTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAA  >  NC_000913/2732342‑2732595                                                                                                                                     |                                                                                                                          ttCCCCACACCAGTTGGGCCGAGGAACAGGAACGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTc                                                                                                                    <  1:742266/140‑1 (MQ=255)  tCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCt                                                                                                                   >  1:215660/1‑140 (MQ=255)   ccccACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCtt                                                                                                                  <  1:129995/140‑1 (MQ=255)    cccACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTg                                                                                                                 >  2:262383/1‑140 (MQ=255)         acCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCa                                                                                                            >  2:508445/1‑140 (MQ=255)           cAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATa                                                                                                          <  1:63893/140‑1 (MQ=255)              ttGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACgc                                                                                                       >  2:121688/1‑140 (MQ=255)                   ccGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGcagcag                                                                                                  >  2:794291/1‑140 (MQ=255)                   ccGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGcagcag                                                                                                  >  1:487464/1‑140 (MQ=255)                              gAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGcgct                                                                                       <  2:732579/140‑1 (MQ=255)                              gAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGcgct                                                                                       <  2:406827/140‑1 (MQ=255)                              gAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGcgct                                                                                       <  2:197964/140‑1 (MQ=255)                                     ccAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTc                                                                                >  2:413049/1‑140 (MQ=255)                                        aTCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCcat                                                                             >  2:388091/1‑140 (MQ=255)                                            ggCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATg                                                                         <  1:508445/140‑1 (MQ=255)                                             gCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGc                                                                        >  2:176472/1‑140 (MQ=255)                                                       tCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCACCCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCgg                                                              <  2:435536/140‑1 (MQ=255)                                                          gCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAt                                                           >  1:350204/1‑140 (MQ=255)                                                           ccAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATc                                                          >  1:372282/1‑140 (MQ=255)                                                            cAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATcc                                                         <  2:350204/140‑1 (MQ=255)                                                                    cACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCaa                                                 <  1:740324/140‑1 (MQ=255)                                                                     aCGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAAc                                                >  1:8327/1‑140 (MQ=255)                                                                         cTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCgg                                                              >  2:587241/1‑122 (MQ=255)                                                                         cTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCgg                                                              <  1:587241/122‑1 (MQ=255)                                                                            cgacgaATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAg                                         <  2:372282/140‑1 (MQ=255)                                                                            cgacgaATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAg                                         <  2:556259/140‑1 (MQ=255)                                                                            cgacgaATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAg                                         <  1:121688/140‑1 (MQ=255)                                                                                        ttAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAGCACTTCAGCAAt                             >  1:221340/1‑140 (MQ=255)                                                                                                cGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGt                     >  2:322341/1‑140 (MQ=255)                                                                                                    ccAACCGCTTCGTTCTGACCAACTACGCGATGGCGCAGTTCTGGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGt                 <  2:678169/139‑1 (MQ=255)                                                                                                                   tGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCaa  <  1:250130/140‑1 (MQ=255)                                                                                                                   tGACCAATTACGCGATGGCGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCaa  <  1:176472/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                          TTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAA  >  NC_000913/2732342‑2732595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑