Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,931	T→C	N35S (AAC→AGC)	pgeF ←	polyphenol oxidase YfiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,931	0	T	C	100.0%	90.6 / NA	28	N35S (AAC→AGC)	pgeF	polyphenol oxidase YfiH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/14);  total (14/14)

GCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACTCATAACCAGTCCACTTCATCCTTA  >  NC_000913/2734795‑2735055                                                                                                                                         |                                                                                                                             gCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCtga                                                                                                                           >  2:142585/1‑140 (MQ=255)  ccAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCtgag                                                                                                                          <  1:3180/140‑1 (MQ=255)  ccAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCtgag                                                                                                                          <  1:576534/140‑1 (MQ=255)           ttAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATa                                                                                                                 <  1:142585/140‑1 (MQ=255)             aaGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACg                                                                                                               >  1:125474/1‑140 (MQ=255)                          ccGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCAc                                                                                                  >  2:123137/1‑140 (MQ=255)                               tACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCAcgccgc                                                                                             >  2:369629/1‑140 (MQ=255)                                   tGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGAt                                                                                         <  2:125474/140‑1 (MQ=255)                                                    tttAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAgg                                                                        >  2:703000/1‑140 (MQ=255)                                                    tttAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAgg                                                                        >  2:570542/1‑140 (MQ=255)                                                         aaGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCa                                                                   >  1:786116/1‑140 (MQ=255)                                                            gCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAaca                                                                <  1:703000/140‑1 (MQ=255)                                                                      cGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCt                                                      >  1:431023/1‑140 (MQ=255)                                                                         agcaAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCg                                                   >  1:627488/1‑140 (MQ=255)                                                                           caAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGc                                                 <  1:123137/140‑1 (MQ=255)                                                                           caAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGc                                                 <  1:570542/140‑1 (MQ=255)                                                                              aaaGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCAc                                              >  1:735096/1‑140 (MQ=255)                                                                                aGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTg                                            <  2:431023/140‑1 (MQ=255)                                                                                              ctcctcAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCt                              <  1:291946/140‑1 (MQ=255)                                                                                              ctcctcAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCt                              <  2:544339/140‑1 (MQ=255)                                                                                                  tcAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACt                          >  2:423674/1‑140 (MQ=255)                                                                                                      cGTGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACTCATa                      >  1:231420/1‑140 (MQ=255)                                                                                                        tGATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACTCATAAc                    <  1:369629/140‑1 (MQ=255)                                                                                                         gATCCGGGTTATCGCCACAATGGGCACCGAGGCTGAGTGAGCCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACTCATAAcc                   >  1:596343/1‑140 (MQ=255)                                                                                                                gTTATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACTCATAACCAGTCCAc            >  2:16082/1‑140 (MQ=255)                                                                                                                 ttATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACTCATAACCAGTCCACt           <  1:16082/140‑1 (MQ=255)                                                                                                                  tATCGCCACAATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACTCATAACCAGTCCACtt          <  1:672289/140‑1 (MQ=255)                                                                                                                          caATGGGCACCGAGGCTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACTCATAACCAGTCCACTTCATCCTTa  <  2:627488/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                             GCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGCGATTCTCCTCAACGTGATCCGGGTTATCGCCACAATGGGCACCGAGGTTGAGTGAGTCATACGGGGGCAAGCTCACGCCGCCGATACGAGTGGAGCTACAGGCCGCAACACCTTTTGGCTGCGGCCACTGCGGGACAATCAGCTTACTCATAACCAGTCCACTTCATCCTTA  >  NC_000913/2734795‑2735055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑